Now showing items 1-12 of 12

Astaxanthin production from Haematococcus pluvialis: effects of light wavelength and salinity

González-Delgado, Angel Darío; Barajas Solano, andres F; Torres, Luz Karime; SUAREZ GELVEZ, JOHN HERMOGENES; Urbina-Suarez, Nestor Andres (Contemporary Engineering SciencesBulgaria, 2017-12-28)

Haematococcus pluvialis has attracted much global attention due to its ability to produce huge amounts of astaxanthin, a carotenoid pigment widely applied in pharmaceutical, cosmetic and food industries. In this work, ...
Bioremediation of Aquaculture Wastewater Using Microalgae Chlorella vulgaris

Blanco, Estefany; González-Delgado, Angel Darío; García-Martinez, Janet; Sanchez-Galvis, Edwar; Barajas Solano, andres F (Contemporary Engineering SciencesBulgaria, 2017-12-24)

Microalgae have received increasing attentions as an alternative treatment approach to remediate wastewater for both nutrient removal and biomass production. Wastewater from aquaculture industry contains high levels of ...
Cultivation of Chlorella vulgaris in Aquaculture Wastewater for Protein Production

Blanco, Estefany; Sanchez-Galvis, Edwar; González-Delgado, Angel Darío; García-Martinez, Janet; Barajas Solano, andres F (Contemporary Engineering SciencesColombia, 2018-02-02)

Microalgae have emerged as environment friendly alternative source of valuable products for energy, pharmaceutical and cosmetic industries. These microorganisms have been also studied in wastewater treatments due to its ...
Development of a Selective Method for Metabolites Extraction from Microalgae Biomass

Barajas Solano, andres F; González-Delgado, Angel Darío; Urbina-Suarez, Nestor Andres; Barajas, Crisóstomo; Sanguino, Paola Andrea (Indian Journal of Science and TechnologyIndia, 2018-02)

Background: The production of biofuel and high value products from microalgae exhibits difficulties that have been widely studied to develop viable, efficient and economic methods for recovering metabolites. Objectives: ...
Effect of flocculation on lipid extraction from Chlorella Vulgaris UTEX 1803 using response surface methodology

González-Delgado, Angel Darío; Barajas Solano, andres F; ZUORRO, Antonio; Mena Lopez, Nelson Osvaldo; Ortegón Diaz, Manuel Alejandro; Garcia, Janet Bibiana; Lavecchia, Roberto (Journal of Engineering Science and TechnologyMalasia, 2019-02)

Microalgae are an attractive source of metabolites, such as lipids, proteins, pigments and carbohydrates, of potential interest to the pharmaceutical, food and energy sectors. The aim of this study was to evaluate the ...
Effect of Flocculation Technology on Lipids, Carbohydrates and Proteins and Extraction from Chlorella vulgaris

Sanguino, Paola Andrea; González-Delgado, Angel Darío; Barajas Solano, andres F (Contemporary Engineering SciencesBulgaria, 2018-03-03)

Nowadays, Chlorella vulgaris has emerged as an attractive source of metabolites such as lipids, proteins and carbohydrates, which are widely applied to obtain high value products. This work is focused on evaluating the ...
The Effects of Wavelength and Salinity on Biomass Production from Haematococcus pluvialis

Torres, Luz Karime; González-Delgado, Angel Darío; Barajas Solano, andres F; Urbina-Suarez, Nestor Andres (Contemporary Engineering SciencesBulgaria, 2017-12-24)

Currently, microalgae have emerged as promising source of high-value products due to its biomass productivity, high photosynthetic efficiency and non-competitive environment for growth. In this work, biomass production ...
Evaluation of In-Situ Transient Simultaneous Cell Disruption and Transesterification of Microalgae

González-Delgado, Angel Darío; Barajas Solano, andres F; Peralta-Ruiz, Yeimmy (Contemporary Engineering SciencesBulgaria, 2017-12-07)

Microalgae has recently been highlighted as source of valuable products including biofuel. The production process of biofuels from microalgae involves mass cultivation, harvesting, deep dewatering, lipid extraction and ...
Evaluation of the effect of flocculation on harvesting of microalgaenannochloropsis SP

ZUORRO, Antonio; Barajas Solano, andres F; Mena, Nelson Osvaldo; González-Delgado, Angel Darío; Ortegón, Manuel Alejandro; Sanguino, Paola Andrea; Lavecchia, Roberto (ARPN Journal of Engineering and Applied Sciences, 2018-08)

Microalgae have recently emerged as an attractive source of products for the food, energy and pharmaceutical industries due to their high biomass productivity, metabolic versatility and environmental sustainability. This ...
Improvement of Biorefinery Efficiency for Microalgae Nannochloropsis sp. via Harvesting Technology Evaluation

Sanguino, Paola Andrea; González-Delgado, Angel Darío; Barajas Solano, andres F (Contemporary Engineering SciencesBulgaria, 2018-04-04)

Recently, microalgal biomass has attached much attention due to the wide diversity of compounds synthesized from different metabolic pathways. This work attempts to study metabolites recovery from Nannochloropsis sp. ...
S-curve and landscape maps for the analysis of trends on industrial textile wastewater treatment

Urbina-Suarez, Nestor Andres; Angel-Ospina, Astrid C.; Lopez-Barrera, German Luciano; Barajas Solano, andres F; Machuca-Martínez, Fiderman (Environmental Advances, 2024-01-18)

The textile industry is one of the most important and economically significant industries in the world. China is the largest textile producer, for countries such as India, Pakistan, Bangladesh and Malaysia this sector is ...
Technical-economic Prefeasibility Study of Astaxanthin Production System from H. pluvial Microalgae in Colombia

González-Delgado, Angel Darío; Barajas Solano, andres F; Barajas, Crisostomo; Morales, J.; Villabona, R. (Indian Journal of Science and TechnologyIndia, 2018-09)

Nowadays, microalgae has been considered as source of valuable products such as astaxanthin, which is a carotenoid with great commercial potential as antioxidant widely used in many industries such as pharmaceutical, ...

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Astaxanthin production from Haematococcus pluvialis: effects of light wavelength and salinity
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González-Delgado, Angel Darío | 2017-12-28

Haematococcus pluvialis has attracted much global attention due to its ability to produce huge amounts of astaxanthin, a carotenoid pigment widely applied in pharmaceutical, cosmetic and food industries. In this work, production of astaxanthin from Haematococcus pluvialis was performed under three different salt concentrations (0, 0.45 and 0.9% v/v) and wavelengths (blue, white and red) in order to evaluate the effects of salinity and light wavelengths on microalgae growth. A multifactorial 32 experimental design was used and its results were studied by Manova analyzer with 95% of reliability. According to the results, the highest amount of astaxanthin (9.72 μg/mL) was obtained at 0.45 %v/v of salt concentration and blue wavelength.

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Bioremediation of Aquaculture Wastewater Using Microalgae Chlorella vulgaris
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Blanco, Estefany | 2017-12-24

Microalgae have received increasing attentions as an alternative treatment approach to remediate wastewater for both nutrient removal and biomass production. Wastewater from aquaculture industry contains high levels of nitrogen and phosphorus, which affect plant growth. Conventional methods for treating aquaculture wastewater generally are inefficient and unprofitable. In this work, microalgae Chlorella vulgaris growth was studied in aquaculture effluent medium in order to reduce its contents of NO3 and PO4. Furthermore, the effect of NaHCO3 and Na2CO3 concentrations and addition time on biomass productivity was evaluate to determine the most suitable conditions for biomass growth. It was found that highest biomass content (0.3 g/L) was achieved at 3.4 g/L of sodium bicarbonate concentration and 19 h of addition time.

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Cultivation of Chlorella vulgaris in Aquaculture Wastewater for Protein Production
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Blanco, Estefany | 2018-02-02

Microalgae have emerged as environment friendly alternative source of valuable products for energy, pharmaceutical and cosmetic industries. These microorganisms have been also studied in wastewater treatments due to its ability to remove CO2, nitrogen, phosphorus, and toxic metals. In this work, cultivation of microalgae Chlorella vulgaris was carried out in aquaculture wastewater in order to reduce its contents of NO3 and PO4. In addition, different concentration of inorganic carbon sources (NaHCO3 and Na2CO3) and addition times were considered for determining suitable conditions in microalgae culture to produce proteins. It was found that highest protein content (45 % w/w) was achieved at 3.4 g/L of NaHCO3 and 19 h of addition time.

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Development of a Selective Method for Metabolites Extraction from Microalgae Biomass
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Barajas Solano, andres F | 2018-02

Background: The production of biofuel and high value products from microalgae exhibits difficulties that have been widely studied to develop viable, efficient and economic methods for recovering metabolites. Objectives: This work is focused on evaluating experimental methods to obtain carbohydrates, proteins and lipids by varying process variables (solvent concentration, temperature, biomass/solvent ratio and moisture content). Methods/Analysis: Carbohydrate and proteins were extracted by acid and alkaline hydrolysis to study the effect of biomass moisture on recovery of these metabolites. Lipids were obtained using hexane and methanol-chloroform methods and its quantification was performed by gravimetric analysis. Findings: It was found that 41.96% and 49.77% of carbohydrates were recovered from C. vulgaris using biomass without thermal pretreatment by acid and alkaline hydrolysis, respectively. Regarding to lipid extraction, hexane was used as solvent for recovering 18.22% of lipids from C. vulgaris. In addition, results suggested that dehydrating biomass at 105°C reduces recovery of high value products. Novelty/Improvement: This study proposes a selective method for extracting metabolites, which enhances efficiency of recovery when is carried out under suitable conditions of biomass moisture, time and solvent volume.

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Effect of flocculation on lipid extraction from Chlorella Vulgaris UTEX 1803 using response surface methodology
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González-Delgado, Angel Darío | 2019-02

Microalgae are an attractive source of metabolites, such as lipids, proteins, pigments and carbohydrates, of potential interest to the pharmaceutical, food and energy sectors. The aim of this study was to evaluate the effect of flocculation on lipid extraction from Chlorella Vulgaris. A 33 experimental design was performed with STATISTICA 7.0 software in order to determine the effects of flocculant (AlCl3) addition, pH and time. The best strategy of pH adjustmentflocculant addition was evaluated, followed by lipid extraction under optimal conditions. pH adjustment after the addition of flocculant provided higher flocculation efficiencies (87.2-98.9%) compared to adjustments made before adding the flocculant (67.8-85.9%). Experiments performed according to the experimental design led to a flocculation efficiency of 99.7% when 100 mg/L of AlCl3, pH 7 and a cultivation time of 18.3 days were used. The percentage of a lipid extract from the flocculated biomass was 2.7% and the flocculant did not affect the production of fatty acid methyl esters.

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Effect of Flocculation Technology on Lipids, Carbohydrates and Proteins and Extraction from Chlorella vulgaris
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Sanguino, Paola Andrea | 2018-03-03

Nowadays, Chlorella vulgaris has emerged as an attractive source of metabolites such as lipids, proteins and carbohydrates, which are widely applied to obtain high value products. This work is focused on evaluating the effect of flocculation on metabolites recovery from Chlorella vulgaris microalgae biomass. Carbohydrates were extracted through acid and alkaline hydrolysis in order to identify the efficiency of these treatments for cell wall disruption. Protein quantification was carried out under alkaline conditions and lipids were recovered by solvent extraction with hexane. It was found that carbohydrate recovery was higher using centrifuged biomass (40.55%) than flocculated biomass (24.31%) by acid treatment, while alkaline treatment exhibited yield of 20.37% and 13.19%, respectively. In addition, efficiency of 53.75% and 32.27% in protein recovery was obtained from both centrifuged and flocculated biomass. The highest lipid extraction yield (54.2%) was achieved using acid hydrolysis and hexane solvent route when centrifuged biomass was used as metabolite source. These results suggested that flocculation affect significantly the efficiency of metabolites extraction.

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The Effects of Wavelength and Salinity on Biomass Production from Haematococcus pluvialis
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Torres, Luz Karime | 2017-12-24

Currently, microalgae have emerged as promising source of high-value products due to its biomass productivity, high photosynthetic efficiency and non-competitive environment for growth. In this work, biomass production from Haematococcus pluvialis was studied at three salt concentrations (0, 0.45 and 0.9% v/v) and blue, white and red wavelengths in order to evaluate the effects of these factors on microalgae growth. A 32 experimental design was performed and its results were analyzed by Manova test with 95% of reliability. The results obtained suggest that the best combination for biomass production consists of red wavelength and salt concentration of 0% v/v with a maximum productivity of 8172.2 mg/mL.

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Evaluation of In-Situ Transient Simultaneous Cell Disruption and Transesterification of Microalgae
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González-Delgado, Angel Darío | 2017-12-07

Microalgae has recently been highlighted as source of valuable products including biofuel. The production process of biofuels from microalgae involves mass cultivation, harvesting, deep dewatering, lipid extraction and biofuel conversion. In this work, lipids from microalgae Navicula sp. were obtained using multifunctional process that consists of acid hydrolysis or cellular disruption, oil extraction and in situ transesterification. The effect of alcohol added to produce ethyl and methyl esters on lipid extraction efficiency was evaluated using methanol and ethanol in order to determine the most suitable route for obtaining the high values of lipids and total reducing sugar. The highest lipid extraction efficiency and total reducing sugar (7.72 % and 2.63 mg/ml, respectively) was obtained for methanol. The low lipid extraction efficiency of multifuctional process is due to transesterification of lipids that gradually released into the system. The formation of alkyl esters was confirmed by FTIR with an increase in carbonyl peak as the reaction progressed, thus muntifuctional process reduce cost of alkyl esters production by eliminating the step of lipid extraction by solvent.

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Evaluation of the effect of flocculation on harvesting of microalgaenannochloropsis SP
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ZUORRO, Antonio | 2018-08

Microalgae have recently emerged as an attractive source of products for the food, energy and pharmaceutical industries due to their high biomass productivity, metabolic versatility and environmental sustainability. This work focuses on the effect of flocculation on the harvesting of microalgae Nannochloropsissp. Through a three-factor experimental design. The most suitable conditions for flocculation were determined based on flocculant (AlCl3) dosage, pH and culture time. In addition, flocculant adding-pH adjustment methods were studied and harvesting was performed according to the method that provided the best results. The pH adjustment after flocculant addition showed higher flocculation efficiency (above 90%) compared to the reverse process. It was also found that the microalgae cells exhibited spontaneous sedimentation, suggesting that the use of flocculant is not required for biomass collection.

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Improvement of Biorefinery Efficiency for Microalgae Nannochloropsis sp. via Harvesting Technology Evaluation
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Sanguino, Paola Andrea | 2018-04-04

Recently, microalgal biomass has attached much attention due to the wide diversity of compounds synthesized from different metabolic pathways. This work attempts to study metabolites recovery from Nannochloropsis sp. biomass concentrated by centrifugation and flocculation. Carbohydrates were obtained using acid and alkaline hydrolysis required for cell disruption. Protein extraction was performed after alkaline pretreatment and lipids were recovery by acid hydrolysis- Soxhlet and alkaline hydrolysis- Soxhlet extraction routes. It was found that carbohydrates were recovered by acid hydrolysis in 41 % and 35.39 % for centrifuged and flocculated biomass, respectively, values higher than thus reported using alkaline hydrolysis. For protein extraction, centrifuged biomass exhibited higher recovery yield (55.48%) than flocculated biomass (38.40%). The lipid extraction route that achieved highest yield (43.45%) was acid hydrolysis with HCl followed by Soxhlet extraction with hexane. In addition, statistical analysis by T test suggested that flocculants affect negatively biomass culture, hence, efficiency of metabolites extraction.

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S-curve and landscape maps for the analysis of trends on industrial textile wastewater treatment
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Urbina-Suarez, Nestor Andres | 2024-01-18

The textile industry is one of the most important and economically significant industries in the world. China is the largest textile producer, for countries such as India, Pakistan, Bangladesh and Malaysia this sector is of crucial importance and it is also a growing sector in Latin America. This industry is responsible for 10 % of annual global carbon emissions and 20 % of global wastewater. This figure is worrying as this wastewater is highly recalcitrant, which has led to research into different treatment methods that are efficient and can provide a sustainable solution to this problem. The aim of this literature review was to identify the existing methods for the treatment of dry scrubber wastewater (TWWT) and the effects of this wastewater and its composition, focusing on biological, chemical and physicochemical processes from the point of view of advantages and disadvantages. It was analyzed which are the most important scientific areas of TWWT and which countries are the most important in this field, as well as the primary pollutants and TWWT processes. Finally, this analysis provides a context for current trends in TWWT research. In general, coagulation methods have been found to be fast but present difficulties due to the use of toxic coagulants; advanced oxidation processes (AOPs) are positioned as very effective methods that can disinfect and decontaminate these effluents. Currently, biological systems coupled with AOPs are a growing trend. Microalgae and cyanobacteria processes coupled with AOPs have attracted great interest due to the possibility of obtaining high added value products through the biorefining of biomass.

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Technical-economic Prefeasibility Study of Astaxanthin Production System from H. pluvial Microalgae in Colombia
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González-Delgado, Angel Darío | 2018-09

Nowadays, microalgae has been considered as source of valuable products such as astaxanthin, which is a carotenoid with great commercial potential as antioxidant widely used in many industries such as pharmaceutical, cosmetics and health supplement. Objectives: This work was focused on technical-economic pre-feasibility study of hybrid system for cultivation, harvesting and extraction of astaxanthin from H. pluvialis microalgae in five Colombian cities (Barranquilla, Cartagena, Santa Marta, Barrancabermeja and Cúcuta). Methods/Analysis: A hybrid system was purposed based on flat panel + open pond Photo Bioreactor (PBR), in which cell growth is inhibited by increasing radiation. Additional stages as centrifugation, filtration, and drying, milling and supercritical extraction were also implemented. An economic prefeasibility study was applied in order to determine the suitability of locations in terms of NPV, IRR and PP. Findings: It was found that the most feasible location for astaxanthin production is Santa Marta with NPV, IRR and PP of € 5,529,203, 50% and 1.9 years, respectively. Novelty/Improvement: These results suggested that astaxanthin can satisfy national demand of antioxidants by producing it from H. pluvialis microalgae.

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