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dc.contributor.authorPacheco, S
dc.contributor.authorNiño, Lilibeth
dc.contributor.authorGelves, German
dc.date.accessioned2021-11-27T17:49:56Z
dc.date.available2021-11-27T17:49:56Z
dc.date.issued2020-11-04
dc.identifier.urihttp://repositorio.ufps.edu.co/handle/ufps/1501
dc.description.abstractAnti-thrombin III is a protein performing anticoagulant function by preventing coagulation process and currently it is used in critical Covid-19 patients. In the present research, recombinant anti-thrombin production at large scale is simulated using SuperPro Designer. Special emphasis was analysed for the elucidation of required unit operation at industrial scale. Saccharomyces cerevisiae yeast is fed with 50 g/L carbon source together with 1.9 g/L amino acids and 6.7 g/L nitrogenous yeast base. All yields are taken from current references. The process is divided in two stages: upstream and downstream. In upward flow a total 11.2 g/L of biomass is calculated. Subsequently, anti-thrombin extraction and purification strategies are proposed. Interestingly, a purified anti-thrombin protein is obtained at 312 mg/L. Based on the latter, the large scale plant proposed in this research can reach 26 vials/hour produced at a concentration of 250 mg. Also productivity and prefeasibility are evaluated and annual production of 205,920 anti-thrombin vials is calculated. Therefore, each vial has a production cost of 180 USD. Based on the authors knowledge, information regarding antithrombin large scale trends are scarce. That is why this is the motivation of this research to perform a computational estimate for the large scale operations involved for the anti-thrombin production.eng
dc.format.extent11 páginasspa
dc.format.mimetypeapplication/pdfspa
dc.language.isoengspa
dc.publisherJournal of Physics: Conference Seriesspa
dc.relation.ispartofJournal of Physics: Conference Series ISSN: 1742-6596, 2020 vol:1655 fasc: N/A págs: 1 - 10, DOI:10.1088/1742-6596/1655/1/012081
dc.rightsContent from this work may be used under the terms of theCreative Commons Attribution 3.0 licence. Any further distribution of this work must maintain attribution to the author(s) and the title of the work, journal citation and DOI. Published under licence by IOP Publishing Ltdeng
dc.sourcehttps://iopscience.iop.org/article/10.1088/1742-6596/1655/1/012081spa
dc.titleRecombinant Anti-Thrombin Production from Saccharomyces Cerevisiae: Large Scale Trends Based on Computational Predictionseng
dc.typeArtículo de revistaspa
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dc.identifier.doi10.1088/1742-6596/1655/1/012081
dc.publisher.placeReino Unidospa
dc.relation.citationeditionVol. 1655, 012081 (2020)spa
dc.relation.citationendpage10spa
dc.relation.citationstartpage1spa
dc.relation.citationvolume1655spa
dc.relation.citesPacheco, S., Niño, L. y Gelves, G. (2020). Recombinant anti-thrombin production from saccharomyces cerevisiae: Large scale trends based on computational predictions. Journal of Physics: Conference Series, 1655, Artículo 012081. https://doi.org/10.1088/1742-6596/1655/1/012081
dc.relation.ispartofjournalJournal of Physics: Conference Seriesspa
dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.rights.creativecommonsAtribución 4.0 Internacional (CC BY 4.0)spa
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oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85spa
dc.type.versioninfo:eu-repo/semantics/publishedVersionspa


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