Recombinant Anti-Thrombin Production from Saccharomyces Cerevisiae: Large Scale Trends Based on Computational Predictions
Artículo de revista
2020-11-04
Journal of Physics: Conference Series
Reino Unido
Anti-thrombin III is a protein performing anticoagulant function by preventing
coagulation process and currently it is used in critical Covid-19 patients. In the present
research, recombinant anti-thrombin production at large scale is simulated using SuperPro
Designer. Special emphasis was analysed for the elucidation of required unit operation at
industrial scale. Saccharomyces cerevisiae yeast is fed with 50 g/L carbon source together
with 1.9 g/L amino acids and 6.7 g/L nitrogenous yeast base. All yields are taken from current
references. The process is divided in two stages: upstream and downstream. In upward flow a
total 11.2 g/L of biomass is calculated. Subsequently, anti-thrombin extraction and purification
strategies are proposed. Interestingly, a purified anti-thrombin protein is obtained at 312 mg/L.
Based on the latter, the large scale plant proposed in this research can reach 26 vials/hour
produced at a concentration of 250 mg. Also productivity and prefeasibility are evaluated and
annual production of 205,920 anti-thrombin vials is calculated. Therefore, each vial has a
production cost of 180 USD. Based on the authors knowledge, information regarding antithrombin large scale trends are scarce. That is why this is the motivation of this research to
perform a computational estimate for the large scale operations involved for the anti-thrombin
production.
- Ambiente y Vida - GIAV [124]
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