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dc.contributor.authorSALAZAR MERCADO, SEIR ANTONIO
dc.contributor.authorQuintero Caleño, Jesús David
dc.contributor.authorMoreno Rozo, laura Yolima
dc.date.accessioned2021-11-12T17:16:54Z
dc.date.available2021-11-12T17:16:54Z
dc.date.issued2020-04-03
dc.identifier.urihttp://repositorio.ufps.edu.co/handle/ufps/943
dc.description.abstractThe aim of the present study was to determine the most suitable pretreatment to enhance the tetrazolium test in seeds of the Epidendrum genus. Initially, mature capsules were harvested at El Escorial village, in the municipality of Pamplona, ​​Colombia. Subsequently, the seeds were subjected to five pretreatments: deionized water, 0.5% NaClO, 1.0% NaClO, 10.0% sucrose and a control group. Using the syringe method with cloth filter, the seeds were rinsed with distilled water and subjected to two concentrations of tetrazolium solution (0.25%, 1.0%) and three exposure times (6 h, 24 h and 48 h). Finally, the tetrazolium viability test results were correlated with the in vitro germination test. It was found that the use of deionized water improves the efficiency of the tetrazolium test in seeds of Epidendrum fimbriatum and E. microtum; as in seeds of E. elongatum when using 1.0% tetrazolium for 24 h. Similarly, a high correlation was found between viability and germination, using deionized water and 10.0% sucrose, with homogeneous results with each othereng
dc.description.abstractO objetivo do presente estudo foi determinar o pré-tratamento mais adequado para aprimorar a metodologia do teste de tetrazólio em sementes do gênero Epidendrum. Inicialmente, cápsulas maduras foram coletadas na vila de El Escorial, no município de Pamplona, Colômbia. Em seguida, as sementes foram submetidas a cinco pré-tratamentos: água deionizada, NaClO a 0,5%, NaClO a 1,0%, sacarose a 10,0% e um controle. Utilizando o método de seringa com filtro de pano, as sementes foram lavadas com água destilada e submetidas a duas concentrações de solução de tetrazólio (0,25% e 1,0%) e três tempos de exposição (6 h, 24 h e 48 h). Finalmente, os resultados do teste de viabilidade do tetrazólio foram comparados ao teste de germinação in vitro. Verificou-se que o uso de água deionizada melhora a eficiência do teste de tetrazólio em sementes de Epidendrum fimbriatum e de E. microtum, assim como em sementes de E. elongatum ao usar 1,0% de tetrazólio por 24 h. Da mesma maneira, foi encontrada alta correlação entre viabilidade e germinação, utilizando água deionizada e 10,0% de sacarose, com resultados homogêneos.por
dc.format.mimetypeapplication/pdfspa
dc.language.isoengspa
dc.publisherJournal of Seed Sciencespa
dc.relation.ispartofJournal of Seed Science
dc.rightsThis is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.eng
dc.sourcehttps://www.scielo.br/j/jss/a/zsfDpVLLfmCcndSMZQzHhPM/?lang=enspa
dc.titleImprovement of the methodology of the tetrazolium test using different pretreatments in seeds of the genus Epidendrum (Orchidaceae)eng
dc.typeArtículo de revistaspa
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dc.identifier.doihttps://doi.org/10.1590/2317-1545v42231028
dc.publisher.placeBrasilia,Brasilspa
dc.relation.citationeditionVol.42 No.e202042013.(2020)spa
dc.relation.citationendpage8spa
dc.relation.citationissuee202042013 (2020)spa
dc.relation.citationstartpage1spa
dc.relation.citationvolume42spa
dc.relation.citesMercado, S. A. S., Caleño, J. D. Q., & Rozo, L. Y. M. (2020). Improvement of the methodology of the tetrazolium test using different pretreatments in seeds of the genus Epidendrum (Orchidaceae). Journal of Seed Science, 42.
dc.relation.ispartofjournalJournal of Seed Sciencespa
dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.rights.creativecommonsAtribución 4.0 Internacional (CC BY 4.0)spa
dc.subject.proposalorchideng
dc.subject.proposalsodium hypochloriteeng
dc.subject.proposalsucroseeng
dc.subject.proposalseed viabilityeng
dc.subject.proposalorquidáceapor
dc.subject.proposalhipoclorito de sódiopor
dc.subject.proposalsacarosepor
dc.subject.proposalviabilidade de sementespor
dc.title.translatedAprimoramento da metodologia do teste de tetrazólio utilizando diferentes pré-tratamentos em sementes do gênero Epidendrum (Orchidaceae)
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