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Cytotoxic evaluation of sodium hypochlorite, using Pisum sativum L as effective bioindicator
| dc.contributor.author | SALAZAR MERCADO, SEIR ANTONIO | |
| dc.contributor.author | Torres-León, Carlos Andrés | |
| dc.contributor.author | Rojas Suárez, Jhan Piero | |
| dc.date.accessioned | 2021-11-22T17:27:34Z | |
| dc.date.available | 2021-11-22T17:27:34Z | |
| dc.date.issued | 2019-05-30 | |
| dc.identifier.uri | http://repositorio.ufps.edu.co/handle/ufps/1233 | |
| dc.description | The objective of this study was to evaluate the cytotoxic effect of different sodium hypochlorite concentrations, using apical root cells of P. sativum as a bioindicator. Initially, the seeds of P. sativum were exposed to different concentrations of sodium hypochlorite (0.1, 0.2, 0.4, 0.8, 1.6, 2 mg L−1) and to a control solution based on distilled water. Next, root growth was measured during 24, 48 and 72 h. Subsequently, the mitotic index (MI) and cellular anomalies (5000 cells per treatment) were determined at 72 h. According to the results obtained, a decrease in root growth was observed at concentrations of 0.4, 1.6 and 2 mg L−1. Likewise, it was evident that, among all the evaluated concentrations, an inhibition of mitosis higher than 50% was presented. Additionally, chromosomal anomalies were also generated, such as Nuclear notch, lagging chromosomes and Chromosomal break, which were present in all the concentrations evaluated. In addition, the presence of micronuclei at concentrations of 2.0 and 1.6 mg L−1 indicate that sodium hypochlorite is a highly cytotoxic substance. Therefore, P. sativum is a specie that offers a feasible experimental model to be implemented in the laboratory with the aim to evaluate the cytotoxic effect of any cytotoxic substance. | eng |
| dc.format.extent | 06 páginas | spa |
| dc.format.mimetype | application/pdf | spa |
| dc.language.iso | eng | spa |
| dc.publisher | Ecotoxicology and Environmental Safety | spa |
| dc.relation.ispartof | Ecotoxicology and Environmental Safety | |
| dc.rights | © 2019 Elsevier Inc. All rights reserved. | eng |
| dc.source | https://www.sciencedirect.com/science/article/abs/pii/S0147651319301770 | spa |
| dc.title | Cytotoxic evaluation of sodium hypochlorite, using Pisum sativum L as effective bioindicator | eng |
| dc.type | Artículo de revista | spa |
| dcterms.references | Hoshina M.M. Evaluation of a possible contami-nation of the waters of the Claro RiverMunicipality of Rio Claro, part of the Corumbataí River Basin, with the mutagenicity tests using Allium cepa, Tesis Licenciatura en Ciencias Biológicas. State University of São Paulo, Rio Claro, SP. 2002. | spa |
| dcterms.references | Kotelnikova, A., Fastovets, I., Dmitry, S., Stolbova, V., 2019. Toxicity assay of lanthanum and cerium in solutions and soil. Ecotoxicol. Environ. Safe 167, 20–28. https://doi. org/10.1016/j.ecoenv.2018.09.117. | spa |
| dcterms.references | Liman, R., Acikbas, Y., Ciğerci, İ., 2019. Cytotoxicity and genotoxicity of cerium oxide micro and nanoparticles by Allium and comet tests. Ecotoxicol. Environ. Safe. 168, 408–414. https://doi.org/10.1016/j.ecoenv.2018.10.088. | spa |
| dcterms.references | Silva, M., Yamashita, O., Rossi, A., Concenco, G., Carvalho, M., Felito, R., 2018. Cytotoxic and genotoxic effects of macroptilium lathyroides. Planta Daninha 36. https://doi. org/10.1590/S0100-83582018360100043. | spa |
| dc.identifier.doi | https://doi.org/10.1016/j.ecoenv.2019.02.027 | |
| dc.publisher.place | Estados Unidos | spa |
| dc.relation.citationedition | Vol.173 (2019) | spa |
| dc.relation.citationendpage | 76 | spa |
| dc.relation.citationissue | (2019) | spa |
| dc.relation.citationstartpage | 71 | spa |
| dc.relation.citationvolume | 173 | spa |
| dc.relation.cites | Salazar-Mercado, S. A., Torres-León, C. A., & Rojas-Suárez, J. P. (2019). Cytotoxic evaluation of sodium hypochlorite, using Pisum sativum L as effective bioindicator. Ecotoxicology and environmental safety, 173, 71-76. | |
| dc.relation.ispartofjournal | Ecotoxicology and Environmental Safety | spa |
| dc.rights.accessrights | info:eu-repo/semantics/openAccess | spa |
| dc.rights.creativecommons | Atribución 4.0 Internacional (CC BY 4.0) | spa |
| dc.subject.proposal | Cellular abnormalities | eng |
| dc.subject.proposal | Cytotoxicity | eng |
| dc.subject.proposal | Sodium hypochlorite | eng |
| dc.subject.proposal | Mitotic index | eng |
| dc.subject.proposal | P. sativum | eng |
| dc.type.coar | http://purl.org/coar/resource_type/c_6501 | spa |
| dc.type.content | Text | spa |
| dc.type.driver | info:eu-repo/semantics/article | spa |
| dc.type.redcol | http://purl.org/redcol/resource_type/ART | spa |
| oaire.accessrights | http://purl.org/coar/access_right/c_abf2 | spa |
| oaire.version | http://purl.org/coar/version/c_970fb48d4fbd8a85 | spa |
| dc.type.version | info:eu-repo/semantics/publishedVersion | spa |
