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dc.contributor.authorSALAZAR MERCADO, SEIR ANTONIO
dc.contributor.authorBotello Delgado, Edinson Alexander
dc.contributor.authorQuintero Caleño, Jesús David
dc.date.accessioned2021-11-12T21:02:01Z
dc.date.available2021-11-12T21:02:01Z
dc.date.issued2020-03-17
dc.identifier.urihttp://repositorio.ufps.edu.co/handle/ufps/951
dc.description.abstractOrchids are affected by several factors that impair their spreading, which is necessary to know the viability of their seeds. The aim of this research was to determine the most suitable preconditioning treatment to potentiate the tetrazolium test in Epidendrum barbaricum seeds. Initially, the mature capsules were collected near the city of Pasto in the Department of Nariño (Colombia), and seeds were obtained. Subsequently, the seeds were submitted to four pretreatments: immersion in distilled water, 1% hypochlorite, 10% alcohol and 10% sucrose. Seeds were then rinsed with distilled water and exposed to two concentrations of 2,3,5-trifenyl tetrazolium chloride (0.25%, 1%) and different exposure times (6, 12, 24, and 48 hours). To perform the tests, the 5 ml syringe with cloth filter method was used. The viability test results were corroborated with the in vitro germination test, using the MS (Murashige and Skoog) culture medium. The most reliable viability findings (93%) were obtained by using preconditioning with sodium hypochlorite, a value that has a high correlation with the percentage of germination (93%), independent of the tetrazolium concentration at an exposure time of 24 hourseng
dc.description.abstractLas orquídeas son afectadas por varios factores que perjudican su propagación. En este estudio se evaluaron diferentes métodos de pre-acondicionamento para potenciar la prueba de tetrazolio en semillas de Epidendrum barbaricum. Inicialmente las cápsulas maduras fueron recolectadas en la ciudad de Pasto, departamento de Nariño (Colombia), las cuales fueron sometidas a los pretratamientos: inmersión en agua destilada, hipoclorito al 1%, alcohol al 10% y sacarosa al 10%, antes de ser lavadas con agua destilada y tratadas con concentraciones de 2,3,5- cloruro trifenil tetrazolio (0.25%, y 1%) durante tiempos de exposición de 6, 12, 24 y 48 horas. Para las aplicaciones fueron utilizadas jeringas de 5 ml con filtro de tela. Los resultados del test de viabilidad fueron validados con la prueba de germinación in vitro, utilizando el medio de cultivo MS (Murashige and Skoog). Los mejores porcentajes de viabilidad (93%) se encontraron con la aplicación de hipoclorito de sodio, con una alta correlación con el porcentaje de germinación (93%), independiente de la concentración de tetrazolio y un tiempo de exposición de 24 horasspa
dc.format.mimetypeapplication/pdfspa
dc.language.isoengspa
dc.publisherRevista Acta Agronomicaspa
dc.relation.ispartofRevista Acta Agronomica
dc.rightsEsta obra está bajo una licencia internacional Creative Commons Atribución-NoComercial-SinDerivadas 4.0.spa
dc.sourcehttps://revistas.unal.edu.co/index.php/acta_agronomica/article/view/79619spa
dc.titlePre-treatments effect on the tetrazolium test on Epidendrum barbaricum Hágsater & Dodson seedseng
dc.typeArtículo de revistaspa
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dc.identifier.doihttps://doi.org/10.15446/acag.v68n4.79619
dc.publisher.placeBogota ,Colombiaspa
dc.relation.citationeditionVol.68 No.4.(2019)spa
dc.relation.citationendpage311spa
dc.relation.citationissue4 (2019)spa
dc.relation.citationstartpage306spa
dc.relation.citationvolume68spa
dc.relation.citesSalazar Mercado, S. A., Botello Delgado, E. A., & Quintero Caleño, J. D. (2019). Pre-treatments effect on the tetrazolium test on Epidendrum barbaricum Hágsater & Dodson seeds. Acta Agronómica, 68(4), 306-311.
dc.relation.ispartofjournalRevista Acta Agronomicaspa
dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.rights.creativecommonsAtribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0)spa
dc.subject.proposalCapsuleseng
dc.subject.proposalGerminative capacityeng
dc.subject.proposalOrchidseng
dc.subject.proposalSeeds qualityeng
dc.subject.proposalSodium hypochloriteeng
dc.subject.proposalCapacidad germinativaspa
dc.subject.proposalCalidad de semillasspa
dc.subject.proposalhipoclorito de sodiospa
dc.subject.proposalOrquídeasspa
dc.subject.proposalpreacondicionamientospa
dc.title.translatedEfecto de pretatamientos en la prueba de tetrazolio en semillas de Epidendrum barbaricum Hágsater & Dodson
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oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85spa
dc.type.versioninfo:eu-repo/semantics/publishedVersionspa


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