Pre-treatments effect on the tetrazolium test on Epidendrum barbaricum Hágsater & Dodson seeds
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SALAZAR MERCADO, SEIR ANTONIO | 2020-03-17
Orchids are affected by several factors that impair their spreading, which is necessary to know the viability of their
seeds. The aim of this research was to determine the most suitable preconditioning treatment to potentiate the
tetrazolium test in Epidendrum barbaricum seeds. Initially, the mature capsules were collected near the city of
Pasto in the Department of Nariño (Colombia), and seeds were obtained. Subsequently, the seeds were submitted
to four pretreatments: immersion in distilled water, 1% hypochlorite, 10% alcohol and 10% sucrose. Seeds were
then rinsed with distilled water and exposed to two concentrations of 2,3,5-trifenyl tetrazolium chloride (0.25%,
1%) and different exposure times (6, 12, 24, and 48 hours). To perform the tests, the 5 ml syringe with cloth
filter method was used. The viability test results were corroborated with the in vitro germination test, using the
MS (Murashige and Skoog) culture medium. The most reliable viability findings (93%) were obtained by using
preconditioning with sodium hypochlorite, a value that has a high correlation with the percentage of germination
(93%), independent of the tetrazolium concentration at an exposure time of 24 hours
LEER