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Effect of the medium composition on the asymbiotic germination and in vitro development of the Laeliocattleya hybrid

SALAZAR MERCADO, SEIR ANTONIO; Botello Delgado, Edinson Alexander (South African Journal of Botany, 2020-09-04)

In vitro cultivation techniques are viable alternatives for the germination and production of orchid seedlings in a short period. From another perspective, when studying the optimal germination conditions, knowing about ...
Optimización de la prueba de tetrazolio para evaluar la viabilidad en semillas de Solanum lycopersicum L.

SALAZAR MERCADO, SEIR ANTONIO; Botello Delgado, Edinson Alexander; Quintero Caleño, Jesús David (Corpoica Ciencia y Tecnologia Agropecuaria, 2020-08-31)

El tomate (Solanum lycopersicum) es una de las hortalizas más consumidas en el mundo con un aumento en su demanda, por lo que conocer la viabilidad de sus semillas es de suma importancia, ya que estas son la base del éxito ...
Pre-treatments effect on the tetrazolium test on Epidendrum barbaricum Hágsater & Dodson seeds

SALAZAR MERCADO, SEIR ANTONIO; Botello Delgado, Edinson Alexander; Quintero Caleño, Jesús David (Revista Acta Agronomica, 2020-03-17)

Orchids are affected by several factors that impair their spreading, which is necessary to know the viability of their seeds. The aim of this research was to determine the most suitable preconditioning treatment to potentiate ...

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Effect of the medium composition on the asymbiotic germination and in vitro development of the Laeliocattleya hybrid
...
SALAZAR MERCADO, SEIR ANTONIO | 2020-09-04

In vitro cultivation techniques are viable alternatives for the germination and production of orchid seedlings in a short period. From another perspective, when studying the optimal germination conditions, knowing about the seeds viability is essential. Therefore, this research aimed to study the appropriate medium for germination and in vitro development of xLaeliocattleya Richard Muller. In addition, optimal preconditioning was determined to enhance the tetrazolium test. Initially, the indehiscent capsules were collected in a nursery in Bochalema-Norte de Santander. From them, seeds were obtained for the preconditioning and germination tests. One portion was subjected to different precondition treatments: distilled water, sodium hypochlorite (0.5 1.0%) and sucrose (10%). Afterwards, seeds were rinsed and exposed to different concentrations and exposure periods of tetrazolium. Regarding in vitro cultivation, the commercial medium Orchid Seed Sowing Medium, Murashige & Skoog (MS), and the MS supplemented with coconut water (MSC), pineapple juice (MSP) and gibberellic acid (MSGA3) were evaluated. The highest percentage of viability was obtained by subjecting the seeds to preconditioning with 10% sucrose and keeping them for 24 h in a 0.5% tetrazolium solution. With respect to the MS media used, those enriched with organic supplements proved to be superior to the other treatments since the highest results were achieved both in asymbiotic germination (56 § 2.8%) and in seedlings formation (25.8 § 0.8) with the MSP medium, followed by the MSC medium. In this way, a viable and low-cost alternative is offered for the commercial propagation of xLaeliocattleya Richard Muller.

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Optimización de la prueba de tetrazolio para evaluar la viabilidad en semillas de Solanum lycopersicum L.
...
SALAZAR MERCADO, SEIR ANTONIO | 2020-08-31

El tomate (Solanum lycopersicum) es una de las hortalizas más consumidas en el mundo con un aumento en su demanda, por lo que conocer la viabilidad de sus semillas es de suma importancia, ya que estas son la base del éxito de su producción. Debido a esto, el presente estudio tuvo como objetivo optimizar la prueba de tetrazolio determinando el pretratamiento adecuado para potenciar la prueba. El material vegetal se recolectó de cultivos establecidos en el municipio del Cáchira, departamento de Norte de Santander, Colombia. Las semillas se extrajeron de los frutos maduros y, posteriormente, se expusieron a pretratramientos con hipoclorito de sodio y agua destilada durante 10 minutos, con tres concentraciones de 2, 3, 5- cloruro trifenil tetrazolio (0,25 %, 0,15 % y 0,10 %) y distintos tiempos de exposición (6 h, 12 h y 24 h). Los datos de viabilidad obtenidos se corroboraron mediante la prueba de germinación en toallas de papel húmedas. Los resultados de viabilidad más relacionados con la prueba de germinación se obtuvieron al emplear las concentraciones de 0,25 % y 0,15 %, utilizando tanto el pretratamiento con hipoclorito de sodio como la inmersión en agua destilada.

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Pre-treatments effect on the tetrazolium test on Epidendrum barbaricum Hágsater & Dodson seeds
...
SALAZAR MERCADO, SEIR ANTONIO | 2020-03-17

Orchids are affected by several factors that impair their spreading, which is necessary to know the viability of their seeds. The aim of this research was to determine the most suitable preconditioning treatment to potentiate the tetrazolium test in Epidendrum barbaricum seeds. Initially, the mature capsules were collected near the city of Pasto in the Department of Nariño (Colombia), and seeds were obtained. Subsequently, the seeds were submitted to four pretreatments: immersion in distilled water, 1% hypochlorite, 10% alcohol and 10% sucrose. Seeds were then rinsed with distilled water and exposed to two concentrations of 2,3,5-trifenyl tetrazolium chloride (0.25%, 1%) and different exposure times (6, 12, 24, and 48 hours). To perform the tests, the 5 ml syringe with cloth filter method was used. The viability test results were corroborated with the in vitro germination test, using the MS (Murashige and Skoog) culture medium. The most reliable viability findings (93%) were obtained by using preconditioning with sodium hypochlorite, a value that has a high correlation with the percentage of germination (93%), independent of the tetrazolium concentration at an exposure time of 24 hours.

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Listar Ciencias Agronómicas y Pecuarias - GICAP por autor "15882d2ee554c30b9a0b0fb312572e30"

Effect of the medium composition on the asymbiotic germination and in vitro development of the Laeliocattleya hybrid

Optimización de la prueba de tetrazolio para evaluar la viabilidad en semillas de Solanum lycopersicum L.

Pre-treatments effect on the tetrazolium test on Epidendrum barbaricum Hágsater & Dodson seeds

Envíos recientes

Effect of the medium composition on the asymbiotic germination and in vitro development of the Laeliocattleya hybrid

Optimización de la prueba de tetrazolio para evaluar la viabilidad en semillas de Solanum lycopersicum L.

Pre-treatments effect on the tetrazolium test on Epidendrum barbaricum Hágsater & Dodson seeds

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Listar Ciencias Agronómicas y Pecuarias - GICAP por autor "15882d2ee554c30b9a0b0fb312572e30"

Effect of the medium composition on the asymbiotic germination and in vitro development of the Laeliocattleya hybrid ﻿

Optimización de la prueba de tetrazolio para evaluar la viabilidad en semillas de Solanum lycopersicum L. ﻿

Pre-treatments effect on the tetrazolium test on Epidendrum barbaricum Hágsater & Dodson seeds ﻿

Envíos recientes

Effect of the medium composition on the asymbiotic germination and in vitro development of the Laeliocattleya hybrid

Optimización de la prueba de tetrazolio para evaluar la viabilidad en semillas de Solanum lycopersicum L.

Pre-treatments effect on the tetrazolium test on Epidendrum barbaricum Hágsater & Dodson seeds

Portales Institucionales

Effect of the medium composition on the asymbiotic germination and in vitro development of the Laeliocattleya hybrid

Optimización de la prueba de tetrazolio para evaluar la viabilidad en semillas de Solanum lycopersicum L.

Pre-treatments effect on the tetrazolium test on Epidendrum barbaricum Hágsater & Dodson seeds