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Identificación de genes Cry1 en aislados de Bacillus thuringiensis y su efecto tóxico contra Milax gagates, plaga en lechuga (Lactuca sativa)
| dc.contributor.author | Galvis, Fabian | |
| dc.contributor.author | Moreno Rozo, laura Yolima | |
| dc.date.accessioned | 2021-12-09T21:14:21Z | |
| dc.date.available | 2021-12-09T21:14:21Z | |
| dc.date.issued | 2018-08 | |
| dc.identifier.uri | http://repositorio.ufps.edu.co/handle/ufps/1782 | |
| dc.description.abstract | Milax gagates is one of the main pests in the cultivation of lettuce, causing considerable economic losses. Control with molluscicides totally or partially eliminates the pest but generates a negative impact on the environment due to their high toxicity. Biological management also allows controlling the pest, but in a specific and safe way for the environment. Therefore, the objective of this work was to evaluate the toxic effect of Bacillus thuringiensis, with Cry1 genes, against M. gagates by means of a bioassay. Seventy-five soil samples were taken from several municipalities in Norte de Santander, Colombia, from where 58 isolated colonies of B. thuringiensis with similar characteristics were obtained. Of these colonies, five showed the presence of Cry1 genes, and were those used in the bioassays together with the control B. thuringiensis var. Kurstaki. The treatments caused 90-100 % lethality to M. gagates at a concentration of 500 μg∙mL-1. The calculated LD99 suggests the use of 820 μg∙mL-1 of the biopreparate to obtain 100 % lethality with any of the five isolates studied. | eng |
| dc.description.abstract | Milax gagates constituye una de las principales plagas en el cultivo de lechuga causando considerables pérdidas económicas. El control con molusquicidas elimina total o parcialmente la plaga, pero genera un impacto negativo en el ambiente por su alta toxicidad. El manejo biológico también permite el control de la plaga, pero de manera específica e inocua para el ambiente. Por ello, el objetivo de este trabajo fue evaluar el efecto tóxico de Bacillus thuringiensis, con genes Cry1, contra M. gagates mediante la elaboración de un bioensayo. Se tomaron 75 muestras de suelo de varios municipios del Norte de Santander, Colombia, de donde se obtuvieron 58 colonias aisladas de B. thuringiensis con características similares. De estas colonias, cinco evidenciaron la presencia de genes Cry1, y fueron las empleadas en los bioensayos junto con el control B. thuringiensis var. Kurstaki. Los tratamientos presentaron letalidad ante M. gagates de entre 90 y 100 % a una concentración de 500 μg∙mL-1. La CL99 calculada sugiere el uso de 820 μg∙mL-1 del biopreparado para obtener 100 % de letalidad con cualquiera de los cinco aislados estudiados | spa |
| dc.format.mimetype | application/pdf | spa |
| dc.language.iso | eng | spa |
| dc.publisher | Revista Chapingo Serie Horticultura | spa |
| dc.relation.ispartof | Revista Chapingo Serie Horticultura | |
| dc.rights | This is an open-access article distributed under the terms of the Creative Commons Attribution License view the permissions of this license | eng |
| dc.source | https://revistas.chapingo.mx/horticultura/?section=articles&subsec=issues&numero=255&articulo=2479 | spa |
| dc.title | Identificación de genes Cry1 en aislados de Bacillus thuringiensis y su efecto tóxico contra Milax gagates, plaga en lechuga (Lactuca sativa) | eng |
| dc.type | Artículo de revista | spa |
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| dc.identifier.doi | http://dx.doi.org/10.5154/r.rchsh.2017.07.025 | |
| dc.publisher.place | Ciudad de Mexico , Mexico | spa |
| dc.relation.citationedition | Vol.24 No.2.(2018) | spa |
| dc.relation.citationendpage | 106 | spa |
| dc.relation.citationissue | 2 (2018) | spa |
| dc.relation.citationstartpage | 97 | spa |
| dc.relation.citationvolume | 24 | spa |
| dc.relation.cites | Galvis, F., & Moreno, L. (2018). Identification of Cry1 genes in Bacillus thuringiensis isolates and their toxic effect against Milax gagates, a pest on lettuce (Lactuca sativa). Revista Chapingo. Serie horticultura, 24(2), 97-106. | |
| dc.relation.ispartofjournal | Revista Chapingo Serie Horticultura | spa |
| dc.rights.accessrights | info:eu-repo/semantics/openAccess | spa |
| dc.rights.creativecommons | Atribución 4.0 Internacional (CC BY 4.0) | spa |
| dc.subject.proposal | biological control | eng |
| dc.subject.proposal | slugs | eng |
| dc.subject.proposal | molluscicides | eng |
| dc.subject.proposal | bioassays | eng |
| dc.subject.proposal | control biológico | spa |
| dc.subject.proposal | babosas | spa |
| dc.subject.proposal | molusquicidas | spa |
| dc.subject.proposal | bioensayos | spa |
| dc.title.translated | Identificación de genes Cry1 en aislados de Bacillus thuringiensis y su efecto tóxico contra Milax gagates, plaga en lechuga (Lactuca sativa) | |
| dc.type.coar | http://purl.org/coar/resource_type/c_6501 | spa |
| dc.type.content | Text | spa |
| dc.type.driver | info:eu-repo/semantics/article | spa |
| dc.type.redcol | http://purl.org/redcol/resource_type/ART | spa |
| oaire.accessrights | http://purl.org/coar/access_right/c_abf2 | spa |
| oaire.version | http://purl.org/coar/version/c_970fb48d4fbd8a85 | spa |
| dc.type.version | info:eu-repo/semantics/publishedVersion | spa |
