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Cytotoxic evaluation of sodium hypochlorite, using Pisum sativum L as effective bioindicator

SALAZAR MERCADO, SEIR ANTONIO | 2019-05-30

Cytotoxic evaluation of sodium hypochlorite, using Pisum sativum L as effective bioindicator

SALAZAR MERCADO, SEIR ANTONIO | 2019-05-30

The objective of this study was to evaluate the cytotoxic effect of different sodium hypochlorite concentrations, using apical root cells of P. sativum as a bioindicator. Initially, the seeds of P. sativum were exposed to different concentrations of sodium hypochlorite (0.1, 0.2, 0.4, 0.8, 1.6, 2 mg L−1) and to a control solution based on distilled water. Next, root growth was measured during 24, 48 and 72 h. Subsequently, the mitotic index (MI) and cellular anomalies (5000 cells per treatment) were determined at 72 h. According to the results obtained, a decrease in root growth was observed at concentrations of 0.4, 1.6 and 2 mg L−1. Likewise, it was evident that, among all the evaluated concentrations, an inhibition of mitosis higher than 50% was presented. Additionally, chromosomal anomalies were also generated, such as Nuclear notch, lagging chromosomes and Chromosomal break, which were present in all the concentrations evaluated. In addition, the presence of micronuclei at concentrations of 2.0 and 1.6 mg L−1 indicate that sodium hypochlorite is a highly cytotoxic substance. Therefore, P. sativum is a specie that offers a feasible experimental model to be implemented in the laboratory with the aim to evaluate the cytotoxic effect of any cytotoxic substance.

Evaluation of cytotoxic potential of chlorpyrifos using Lens culinaris Med as efficient bioindicator

SALAZAR MERCADO, SEIR ANTONIO | 2019-11-15

The aim of this study was to evaluate the cytotoxic effect of different concentrations of chlorpyrifos (CPF), using L. culinaris apical cells as a biological indicator. L. culinaris seeds were exposed to different concentrations of chlorpyrifos (0, 1, 3, 5, 7, 8, 10 and 15 mg L−1) and a control solution based on distilled water. Subsequently, root growth was measured during 24, 48 and 72 h. Therefore, the mitotic index (MI) and the number of cellular abnormalities were determined at 72 h. According to the obtained results, a decrease in root size was observed in the concentrations of T5 (8 mg L−1) and T6 (10 mg L−1). On the other hand, it was evidenced that, through all the evaluated concentrations, the inhibition of mitosis in the concentrations of T5 (8 mg L−1), T6 (10 mg L−1) and T7 (15 mg L−1) was greater than 50%. Additionally, a variety of chromosomal abnormalities were reported, such as Micronuclei, sticky chromosomes in anaphase, chromosome disruption, irregular anaphase, nucleus absence, nuclear lesions, chromosomes grouped in metaphase, anaphase bridges, metaphase sticky chromosomes, present in all concentrations evaluated. Consequently, the presence of micronuclei in the concentrations of 8 mg L−1, 10 mg L−1 and 15 mg L−1 indicates that the CPF is a highly cytotoxic substance to L. culinaris. Therefore, L. culinaris is a plant species that offers a feasible experimental model to be implemented in laboratory studies with the purpose to evaluate the cytotoxic effect of pesticides.

Evaluation of cytotoxic potential of chlorpyrifos using Lens culinaris Med as efficient bioindicator

SALAZAR MERCADO, SEIR ANTONIO | 2019-11-15

The aim of this study was to evaluate the cytotoxic effect of different concentrations of chlorpyrifos (CPF), using L. culinaris apical cells as a biological indicator. L. culinaris seeds were exposed to different concentrations of chlorpyrifos (0, 1, 3, 5, 7, 8, 10 and 15 mg L−1) and a control solution based on distilled water. Subsequently, root growth was measured during 24, 48 and 72 h. Therefore, the mitotic index (MI) and the number of cellular abnormalities were determined at 72 h. According to the obtained results, a decrease in root size was observed in the concentrations of T5 (8 mg L−1) and T6 (10 mg L−1). On the other hand, it was evidenced that, through all the evaluated concentrations, the inhibition of mitosis in the concentrations of T5 (8 mg L−1), T6 (10 mg L−1) and T7 (15 mg L−1) was greater than 50%. Additionally, a variety of chromosomal abnormalities were reported, such as Micronuclei, sticky chromosomes in anaphase, chromosome disruption, irregular anaphase, nucleus absence, nuclear lesions, chromosomes grouped in metaphase, anaphase bridges, metaphase sticky chromosomes, present in all concentrations evaluated. Consequently, the presence of micronuclei in the concentrations of 8 mg L−1, 10 mg L−1 and 15 mg L−1 indicates that the CPF is a highly cytotoxic substance to L. culinaris. Therefore, L. culinaris is a plant species that offers a feasible experimental model to be implemented in laboratory studies with the purpose to evaluate the cytotoxic effect of pesticides.