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Optimization of the tetrazolium test in three species of orchids of the Andean forest

SALAZAR MERCADO, SEIR ANTONIO; Quintero Caleño, Jesús David; Rojas Suárez, Jhan Piero (Australian Journal of Crop ScienceAustralia, 2020-05)

In order to improve the cultivation and conservation of orchid species, it is important to assess the physiological quality of orchid seeds. One of the most traditional tests for analysing seed quality is the tetrazolium ...
Pre-treatments effect on the tetrazolium test on Epidendrum barbaricum Hágsater & Dodson seeds

SALAZAR MERCADO, SEIR ANTONIO; Botello Delgado, Edinson Alexander; Quintero Caleño, Jesús David (Revista Acta AgronomicaBogota ,Colombia, 2020-03-17)

Orchids are affected by several factors that impair their spreading, which is necessary to know the viability of their seeds. The aim of this research was to determine the most suitable preconditioning treatment to ...
Pre-treatments effect on the tetrazolium test on Epidendrum barbaricum Hágsater & Dodson seeds

SALAZAR MERCADO, SEIR ANTONIO; Botello Delgado, Edinson Alexander; Quintero Caleño, Jesús David (Revista Acta Agronomica, 2020-03-17)

Orchids are affected by several factors that impair their spreading, which is necessary to know the viability of their seeds. The aim of this research was to determine the most suitable preconditioning treatment to potentiate ...
Pre-treatments effect on the tetrazolium test on Epidendrum barbaricum Hágsater & Dodson seeds

SALAZAR MERCADO, SEIR ANTONIO; Quintero Caleño, Jesús David; Botello Delgado, Edinson Alexander (Acta AgronómicaColombia, 2020-03-17)

Orchids are affected by several factors that impair their spreading, which is necessary to know the viability of their seeds. The aim of this research was to determine the most suitable preconditioning treatment to potentiate ...

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Optimization of the tetrazolium test in three species of orchids of the Andean forest
...
SALAZAR MERCADO, SEIR ANTONIO | 2020-05

In order to improve the cultivation and conservation of orchid species, it is important to assess the physiological quality of orchid seeds. One of the most traditional tests for analysing seed quality is the tetrazolium test, whose effectiveness is known to improve with pre-treatment of the seeds. As a result, this study compared various pre-treatments to determine how efficient they were at optimising the tetrazolium test in seeds belonging to Cyrtochilum aemulun, Elleanthus aurantiacus and Lephantes sp. The experiment was based on five different pre-treatment solutions, after which the seeds were exposed to tetrazolium at two different concentrations and for three different exposure times. First, the seeds were soaked for ten minutes in one of five pre-treatment solutions: chlorine 0.5%, chlorine 1%, deionised water, sucrose solution 10% (w/v) or a control in which no pre-treatment was applied. After pre-treatment, the seeds were soaked in the dark in a tetrazolium solution, either at 0.25% concentration or at 1.0% concentration, and for either 6, 24 or 48 hours. The control seeds exposed to no pre-treatment showed an increase in the viability of Lephantes sp. at significant difference from the pre-treated seeds. Similarly, pre-treatment with 10% sucrose produced an increase in the tetrazolium test’s effectiveness in E.aurantiacus seeds, with significant differences from the control in most treatments. Therefore, the use of sucrose as a pre-treatment is recommended, in addition to increasing the concentration and exposure time of the seeds.

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Pre-treatments effect on the tetrazolium test on Epidendrum barbaricum Hágsater & Dodson seeds
...
SALAZAR MERCADO, SEIR ANTONIO | 2020-03-17

Orchids are affected by several factors that impair their spreading, which is necessary to know the viability of their seeds. The aim of this research was to determine the most suitable preconditioning treatment to potentiate the tetrazolium test in Epidendrum barbaricum seeds. Initially, the mature capsules were collected near the city of Pasto in the Department of Nariño (Colombia), and seeds were obtained. Subsequently, the seeds were submitted to four pretreatments: immersion in distilled water, 1% hypochlorite, 10% alcohol and 10% sucrose. Seeds were then rinsed with distilled water and exposed to two concentrations of 2,3,5-trifenyl tetrazolium chloride (0.25%, 1%) and different exposure times (6, 12, 24, and 48 hours). To perform the tests, the 5 ml syringe with cloth filter method was used. The viability test results were corroborated with the in vitro germination test, using the MS (Murashige and Skoog) culture medium. The most reliable viability findings (93%) were obtained by using preconditioning with sodium hypochlorite, a value that has a high correlation with the percentage of germination (93%), independent of the tetrazolium concentration at an exposure time of 24 hours

LEER
Pre-treatments effect on the tetrazolium test on Epidendrum barbaricum Hágsater & Dodson seeds
...
SALAZAR MERCADO, SEIR ANTONIO | 2020-03-17

Orchids are affected by several factors that impair their spreading, which is necessary to know the viability of their seeds. The aim of this research was to determine the most suitable preconditioning treatment to potentiate the tetrazolium test in Epidendrum barbaricum seeds. Initially, the mature capsules were collected near the city of Pasto in the Department of Nariño (Colombia), and seeds were obtained. Subsequently, the seeds were submitted to four pretreatments: immersion in distilled water, 1% hypochlorite, 10% alcohol and 10% sucrose. Seeds were then rinsed with distilled water and exposed to two concentrations of 2,3,5-trifenyl tetrazolium chloride (0.25%, 1%) and different exposure times (6, 12, 24, and 48 hours). To perform the tests, the 5 ml syringe with cloth filter method was used. The viability test results were corroborated with the in vitro germination test, using the MS (Murashige and Skoog) culture medium. The most reliable viability findings (93%) were obtained by using preconditioning with sodium hypochlorite, a value that has a high correlation with the percentage of germination (93%), independent of the tetrazolium concentration at an exposure time of 24 hours.

LEER
Pre-treatments effect on the tetrazolium test on Epidendrum barbaricum Hágsater & Dodson seeds
...
SALAZAR MERCADO, SEIR ANTONIO | 2020-03-17

Orchids are affected by several factors that impair their spreading, which is necessary to know the viability of their seeds. The aim of this research was to determine the most suitable preconditioning treatment to potentiate the tetrazolium test in Epidendrum barbaricum seeds. Initially, the mature capsules were collected near the city of Pasto in the Department of Nariño (Colombia), and seeds were obtained. Subsequently, the seeds were submitted to four pretreatments: immersion in distilled water, 1% hypochlorite, 10% alcohol and 10% sucrose. Seeds were then rinsed with distilled water and exposed to two concentrations of 2,3,5-trifenyl tetrazolium chloride (0.25%, 1%) and different exposure times (6, 12, 24, and 48 hours). To perform the tests, the 5 ml syringe with cloth filter method was used. The viability test results were corroborated with the in vitro germination test, using the MS (Murashige and Skoog) culture medium. The most reliable viability findings (93%) were obtained by using preconditioning with sodium hypochlorite, a value that has a high correlation with the percentage of germination (93%), independent of the tetrazolium concentration at an exposure time of 24 hours.

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Listar por tema "Orchids"

Optimization of the tetrazolium test in three species of orchids of the Andean forest

Pre-treatments effect on the tetrazolium test on Epidendrum barbaricum Hágsater & Dodson seeds

Pre-treatments effect on the tetrazolium test on Epidendrum barbaricum Hágsater & Dodson seeds

Pre-treatments effect on the tetrazolium test on Epidendrum barbaricum Hágsater & Dodson seeds

Envíos recientes

Optimization of the tetrazolium test in three species of orchids of the Andean forest

Pre-treatments effect on the tetrazolium test on Epidendrum barbaricum Hágsater & Dodson seeds

Pre-treatments effect on the tetrazolium test on Epidendrum barbaricum Hágsater & Dodson seeds

Pre-treatments effect on the tetrazolium test on Epidendrum barbaricum Hágsater & Dodson seeds

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Listar por tema "Orchids"

Optimization of the tetrazolium test in three species of orchids of the Andean forest ﻿

Pre-treatments effect on the tetrazolium test on Epidendrum barbaricum Hágsater & Dodson seeds ﻿

Pre-treatments effect on the tetrazolium test on Epidendrum barbaricum Hágsater & Dodson seeds ﻿

Pre-treatments effect on the tetrazolium test on Epidendrum barbaricum Hágsater & Dodson seeds ﻿

Envíos recientes

Optimization of the tetrazolium test in three species of orchids of the Andean forest

Pre-treatments effect on the tetrazolium test on Epidendrum barbaricum Hágsater & Dodson seeds

Pre-treatments effect on the tetrazolium test on Epidendrum barbaricum Hágsater & Dodson seeds

Pre-treatments effect on the tetrazolium test on Epidendrum barbaricum Hágsater & Dodson seeds

Portales Institucionales

Optimization of the tetrazolium test in three species of orchids of the Andean forest

Pre-treatments effect on the tetrazolium test on Epidendrum barbaricum Hágsater & Dodson seeds

Pre-treatments effect on the tetrazolium test on Epidendrum barbaricum Hágsater & Dodson seeds

Pre-treatments effect on the tetrazolium test on Epidendrum barbaricum Hágsater & Dodson seeds