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Development of a Selective Method for Metabolites Extraction from Microalgae Biomass

Barajas Solano, andres F; González-Delgado, Angel Darío; Urbina-Suarez, Nestor Andres; Barajas, Crisóstomo; Sanguino, Paola Andrea (Indian Journal of Science and TechnologyIndia, 2018-02)

Background: The production of biofuel and high value products from microalgae exhibits difficulties that have been widely studied to develop viable, efficient and economic methods for recovering metabolites. Objectives: ...
Effect of flocculation on lipid extraction from Chlorella Vulgaris UTEX 1803 using response surface methodology

González-Delgado, Angel Darío; Barajas Solano, andres F; ZUORRO, Antonio; Mena Lopez, Nelson Osvaldo; Ortegón Diaz, Manuel Alejandro; Garcia, Janet Bibiana; Lavecchia, Roberto (Journal of Engineering Science and TechnologyMalasia, 2019-02)

Microalgae are an attractive source of metabolites, such as lipids, proteins, pigments and carbohydrates, of potential interest to the pharmaceutical, food and energy sectors. The aim of this study was to evaluate the ...
Effect of Flocculation Technology on Lipids, Carbohydrates and Proteins and Extraction from Chlorella vulgaris

Sanguino, Paola Andrea; González-Delgado, Angel Darío; Barajas Solano, andres F (Contemporary Engineering SciencesBulgaria, 2018-03-03)

Nowadays, Chlorella vulgaris has emerged as an attractive source of metabolites such as lipids, proteins and carbohydrates, which are widely applied to obtain high value products. This work is focused on evaluating the ...
Evaluation of the effect of flocculation on harvesting of microalgaenannochloropsis SP

ZUORRO, Antonio; Barajas Solano, andres F; Mena, Nelson Osvaldo; González-Delgado, Angel Darío; Ortegón, Manuel Alejandro; Sanguino, Paola Andrea; Lavecchia, Roberto (ARPN Journal of Engineering and Applied Sciences, 2018-08)

Microalgae have recently emerged as an attractive source of products for the food, energy and pharmaceutical industries due to their high biomass productivity, metabolic versatility and environmental sustainability. This ...

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Development of a Selective Method for Metabolites Extraction from Microalgae Biomass
...
Barajas Solano, andres F | 2018-02

Background: The production of biofuel and high value products from microalgae exhibits difficulties that have been widely studied to develop viable, efficient and economic methods for recovering metabolites. Objectives: This work is focused on evaluating experimental methods to obtain carbohydrates, proteins and lipids by varying process variables (solvent concentration, temperature, biomass/solvent ratio and moisture content). Methods/Analysis: Carbohydrate and proteins were extracted by acid and alkaline hydrolysis to study the effect of biomass moisture on recovery of these metabolites. Lipids were obtained using hexane and methanol-chloroform methods and its quantification was performed by gravimetric analysis. Findings: It was found that 41.96% and 49.77% of carbohydrates were recovered from C. vulgaris using biomass without thermal pretreatment by acid and alkaline hydrolysis, respectively. Regarding to lipid extraction, hexane was used as solvent for recovering 18.22% of lipids from C. vulgaris. In addition, results suggested that dehydrating biomass at 105°C reduces recovery of high value products. Novelty/Improvement: This study proposes a selective method for extracting metabolites, which enhances efficiency of recovery when is carried out under suitable conditions of biomass moisture, time and solvent volume.

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Effect of flocculation on lipid extraction from Chlorella Vulgaris UTEX 1803 using response surface methodology
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González-Delgado, Angel Darío | 2019-02

Microalgae are an attractive source of metabolites, such as lipids, proteins, pigments and carbohydrates, of potential interest to the pharmaceutical, food and energy sectors. The aim of this study was to evaluate the effect of flocculation on lipid extraction from Chlorella Vulgaris. A 33 experimental design was performed with STATISTICA 7.0 software in order to determine the effects of flocculant (AlCl3) addition, pH and time. The best strategy of pH adjustmentflocculant addition was evaluated, followed by lipid extraction under optimal conditions. pH adjustment after the addition of flocculant provided higher flocculation efficiencies (87.2-98.9%) compared to adjustments made before adding the flocculant (67.8-85.9%). Experiments performed according to the experimental design led to a flocculation efficiency of 99.7% when 100 mg/L of AlCl3, pH 7 and a cultivation time of 18.3 days were used. The percentage of a lipid extract from the flocculated biomass was 2.7% and the flocculant did not affect the production of fatty acid methyl esters.

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Effect of Flocculation Technology on Lipids, Carbohydrates and Proteins and Extraction from Chlorella vulgaris
...
Sanguino, Paola Andrea | 2018-03-03

Nowadays, Chlorella vulgaris has emerged as an attractive source of metabolites such as lipids, proteins and carbohydrates, which are widely applied to obtain high value products. This work is focused on evaluating the effect of flocculation on metabolites recovery from Chlorella vulgaris microalgae biomass. Carbohydrates were extracted through acid and alkaline hydrolysis in order to identify the efficiency of these treatments for cell wall disruption. Protein quantification was carried out under alkaline conditions and lipids were recovered by solvent extraction with hexane. It was found that carbohydrate recovery was higher using centrifuged biomass (40.55%) than flocculated biomass (24.31%) by acid treatment, while alkaline treatment exhibited yield of 20.37% and 13.19%, respectively. In addition, efficiency of 53.75% and 32.27% in protein recovery was obtained from both centrifuged and flocculated biomass. The highest lipid extraction yield (54.2%) was achieved using acid hydrolysis and hexane solvent route when centrifuged biomass was used as metabolite source. These results suggested that flocculation affect significantly the efficiency of metabolites extraction.

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Evaluation of the effect of flocculation on harvesting of microalgaenannochloropsis SP
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ZUORRO, Antonio | 2018-08

Microalgae have recently emerged as an attractive source of products for the food, energy and pharmaceutical industries due to their high biomass productivity, metabolic versatility and environmental sustainability. This work focuses on the effect of flocculation on the harvesting of microalgae Nannochloropsissp. Through a three-factor experimental design. The most suitable conditions for flocculation were determined based on flocculant (AlCl3) dosage, pH and culture time. In addition, flocculant adding-pH adjustment methods were studied and harvesting was performed according to the method that provided the best results. The pH adjustment after flocculant addition showed higher flocculation efficiency (above 90%) compared to the reverse process. It was also found that the microalgae cells exhibited spontaneous sedimentation, suggesting that the use of flocculant is not required for biomass collection.

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Browsing by Subject "Flocculation"

Development of a Selective Method for Metabolites Extraction from Microalgae Biomass

Effect of flocculation on lipid extraction from Chlorella Vulgaris UTEX 1803 using response surface methodology

Effect of Flocculation Technology on Lipids, Carbohydrates and Proteins and Extraction from Chlorella vulgaris

Evaluation of the effect of flocculation on harvesting of microalgaenannochloropsis SP

Envíos recientes

Development of a Selective Method for Metabolites Extraction from Microalgae Biomass

Effect of flocculation on lipid extraction from Chlorella Vulgaris UTEX 1803 using response surface methodology

Effect of Flocculation Technology on Lipids, Carbohydrates and Proteins and Extraction from Chlorella vulgaris

Evaluation of the effect of flocculation on harvesting of microalgaenannochloropsis SP

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Browsing by Subject "Flocculation"

Development of a Selective Method for Metabolites Extraction from Microalgae Biomass ﻿

Effect of flocculation on lipid extraction from Chlorella Vulgaris UTEX 1803 using response surface methodology ﻿

Effect of Flocculation Technology on Lipids, Carbohydrates and Proteins and Extraction from Chlorella vulgaris ﻿

Evaluation of the effect of flocculation on harvesting of microalgaenannochloropsis SP ﻿

Envíos recientes

Development of a Selective Method for Metabolites Extraction from Microalgae Biomass

Effect of flocculation on lipid extraction from Chlorella Vulgaris UTEX 1803 using response surface methodology

Effect of Flocculation Technology on Lipids, Carbohydrates and Proteins and Extraction from Chlorella vulgaris

Evaluation of the effect of flocculation on harvesting of microalgaenannochloropsis SP

Portales Institucionales

Development of a Selective Method for Metabolites Extraction from Microalgae Biomass

Effect of flocculation on lipid extraction from Chlorella Vulgaris UTEX 1803 using response surface methodology

Effect of Flocculation Technology on Lipids, Carbohydrates and Proteins and Extraction from Chlorella vulgaris

Evaluation of the effect of flocculation on harvesting of microalgaenannochloropsis SP