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Implementación de la prueba de tetrazolio en las semillas de Raphanus sativus L

SALAZAR MERCADO, SEIR ANTONIO; Quintero Caleño, Jesús David; Bustos Urbano, Víctor Jhoel (Facultad De Ciencias BásicasColombia, 2020-08-14)

El Rábano (Raphanus sativus) es una especie de gran importancia por sus propiedades nutricionales, el alto porcentaje de aceite en sus semillas y la gran cantidad de metabolitos aprovechables. La producción de este cultivo ...
Improvement of the methodology of the tetrazolium test using different pretreatments in seeds of the genus Epidendrum (Orchidaceae)

SALAZAR MERCADO, SEIR ANTONIO; Quintero Caleño, Jesús David; Moreno Rozo, laura Yolima (Journal of Seed ScienceBrasil, 2020-04-03)

The aim of the present study was to determine the most suitable pretreatment to enhance the tetrazolium test in seeds of the Epidendrum genus. Initially, mature capsules were harvested at El Escorial village, in the ...
Improvement of the methodology of the tetrazolium test using different pretreatments in seeds of the genus Epidendrum (Orchidaceae)

SALAZAR MERCADO, SEIR ANTONIO; Quintero Caleño, Jesús David; Moreno Rozo, laura Yolima (Journal of Seed ScienceBrasilia,Brasil, 2020-04-03)

The aim of the present study was to determine the most suitable pretreatment to enhance the tetrazolium test in seeds of the Epidendrum genus. Initially, mature capsules were harvested at El Escorial village, in the ...

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Implementación de la prueba de tetrazolio en las semillas de Raphanus sativus L
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SALAZAR MERCADO, SEIR ANTONIO | 2020-08-14

El Rábano (Raphanus sativus) es una especie de gran importancia por sus propiedades nutricionales, el alto porcentaje de aceite en sus semillas y la gran cantidad de metabolitos aprovechables. La producción de este cultivo se ve influenciada por factores como la utilización de semillas de alta o baja calidad. Es por esto que, en este estudio, se evaluó la aplicación del test de tetrazolio como método para determinar la viabilidad en semillas de Raphanus sativus. Inicialmente, se recolectaron semillas producidas en Villa del Rosario, Norte de Santander, Colombia, las cuales fueron expuestas a dos concentraciones (0,5 % y 1,0 %) y dos tiempos de exposición (24 y 48 horas). Posteriormente, fueron observadas en el estereoscopio para determinar el porcentaje de viabilidad, contabilizando las semillas que presentaron intensa coloración roja en su embrión; se obtuvieron un 99 % y 97 % de semillas viables con los tratamientos T2 (0,5 %, 24 h) y T4 (0,5 %, 48 h) respectivamente, no existiendo diferencias estadísticamente significativas entre sí (p ≤ 0,05: Tukey hsd). En comparación con la prueba de germinación, en la que se obtuvo un promedio de 99 %, no presentaron diferencias estadísticamente significativas los tratamientos T2, T3 y T4. Se concluyó con esto que la prueba de viabilidad con tetrazolio es efectiva a concentraciones de 0,5 % y 1,0 %, durante tiempos de exposición de 24 y 48 horas.

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Improvement of the methodology of the tetrazolium test using different pretreatments in seeds of the genus Epidendrum (Orchidaceae)
...
SALAZAR MERCADO, SEIR ANTONIO | 2020-04-03

The aim of the present study was to determine the most suitable pretreatment to enhance the tetrazolium test in seeds of the Epidendrum genus. Initially, mature capsules were harvested at El Escorial village, in the municipality of Pamplona, Colombia. Subsequently, the seeds were subjected to five pretreatments: deionized water, 0.5% NaClO, 1.0% NaClO, 10.0% sucrose and a control group. Using the syringe method with cloth filter, the seeds were rinsed with distilled water and subjected to two concentrations of tetrazolium solution (0.25%, 1.0%) and three exposure times (6 h, 24 h and 48 h). Finally, the tetrazolium viability test results were correlated with the in vitro germination test. It was found that the use of deionized water improves the efficiency of the tetrazolium test in seeds of Epidendrum fimbriatum and E. microtum; as in seeds of E. elongatum when using 1.0% tetrazolium for 24 h. Similarly, a high correlation was found between viability and germination, using deionized water and 10.0% sucrose, with homogeneous results with each other.

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Improvement of the methodology of the tetrazolium test using different pretreatments in seeds of the genus Epidendrum (Orchidaceae)
...
SALAZAR MERCADO, SEIR ANTONIO | 2020-04-03

The aim of the present study was to determine the most suitable pretreatment to enhance the tetrazolium test in seeds of the Epidendrum genus. Initially, mature capsules were harvested at El Escorial village, in the municipality of Pamplona, Colombia. Subsequently, the seeds were subjected to five pretreatments: deionized water, 0.5% NaClO, 1.0% NaClO, 10.0% sucrose and a control group. Using the syringe method with cloth filter, the seeds were rinsed with distilled water and subjected to two concentrations of tetrazolium solution (0.25%, 1.0%) and three exposure times (6 h, 24 h and 48 h). Finally, the tetrazolium viability test results were correlated with the in vitro germination test. It was found that the use of deionized water improves the efficiency of the tetrazolium test in seeds of Epidendrum fimbriatum and E. microtum; as in seeds of E. elongatum when using 1.0% tetrazolium for 24 h. Similarly, a high correlation was found between viability and germination, using deionized water and 10.0% sucrose, with homogeneous results with each other

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Implementación de la prueba de tetrazolio en las semillas de Raphanus sativus L

Improvement of the methodology of the tetrazolium test using different pretreatments in seeds of the genus Epidendrum (Orchidaceae)

Improvement of the methodology of the tetrazolium test using different pretreatments in seeds of the genus Epidendrum (Orchidaceae)

Envíos recientes

Implementación de la prueba de tetrazolio en las semillas de Raphanus sativus L

Improvement of the methodology of the tetrazolium test using different pretreatments in seeds of the genus Epidendrum (Orchidaceae)

Improvement of the methodology of the tetrazolium test using different pretreatments in seeds of the genus Epidendrum (Orchidaceae)

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Listar por tema "viabilidade de sementes"

Implementación de la prueba de tetrazolio en las semillas de Raphanus sativus L ﻿

Improvement of the methodology of the tetrazolium test using different pretreatments in seeds of the genus Epidendrum (Orchidaceae) ﻿

Improvement of the methodology of the tetrazolium test using different pretreatments in seeds of the genus Epidendrum (Orchidaceae) ﻿

Envíos recientes

Implementación de la prueba de tetrazolio en las semillas de Raphanus sativus L

Improvement of the methodology of the tetrazolium test using different pretreatments in seeds of the genus Epidendrum (Orchidaceae)

Improvement of the methodology of the tetrazolium test using different pretreatments in seeds of the genus Epidendrum (Orchidaceae)

Portales Institucionales

Implementación de la prueba de tetrazolio en las semillas de Raphanus sativus L

Improvement of the methodology of the tetrazolium test using different pretreatments in seeds of the genus Epidendrum (Orchidaceae)

Improvement of the methodology of the tetrazolium test using different pretreatments in seeds of the genus Epidendrum (Orchidaceae)