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Impact of Biomass Drying Process on the Extraction Efficiency of C-Phycoerythrin

Vergel Suarez, Ariadna Hazel; García-Martinez, Janet; López Barrera, German Luciano; Barajas Solano, andres F; ZUORRO, Antonio (BioTech, 2023-04-23)

Drying the biomass produced is one of the critical steps to avoid cell degradation; however, its high energy cost is a significant technological barrier to improving this type of bioprocess’s technical and economic ...
Influence of Critical Parameters on the Extraction of Concentrated C-PE from Thermotolerant Cyanobacteria

Vergel-Suarez, Ariadna H.; García-Martinez, Janet; López Barrera, German Luciano; Urbina-Suarez, Nestor Andres; Barajas Solano, andres F (BioTechSuiza, 2024-06-24)

This work aimed to identify the influence of pH, molarity, w/v fraction, extraction time, agitation, and either a sodium (Na2HPO4 ·7H2O-NaH2PO4 ·H2O) or potassium buffer (K2HPO4 -KH2PO4 ) used in the extraction of ...
Production of industrial-interest colorants in microalgae and cyanobacteria: leveraging nutrient dynamics and photoperiod optimization

Contreras Ropero, Jefferson Eduardo; Barajas Solano, andres F; García-Martinez, Janet; Barajas Ferreira, Crisóstomo; Machuca Martínez, Fiderman (Ingeniería y CompetitividadCali- Colombia, 2024-07-15)

The exploration of the biodiversity of cyanobacteria and microalgae holds great promise for obtaining pigments of industrial interest. This study focused on the bioprospecting of these organisms to obtain pigments of ...

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Impact of Biomass Drying Process on the Extraction Efficiency of C-Phycoerythrin
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Vergel Suarez, Ariadna Hazel | 2023-04-23

Drying the biomass produced is one of the critical steps to avoid cell degradation; however, its high energy cost is a significant technological barrier to improving this type of bioprocess’s technical and economic feasibility. This work explores the impact of the biomass drying method of a strain of Potamosiphon sp. on the extraction efficiency of a phycoerythrin-rich protein extract. To achieve the above, the effect of time (12–24 h), temperature (40–70 ◦C), and drying method (convection oven and dehydrator) were determined using an I-best design with a response surface. According to the statistical results, the factors that most influence the extraction and purity of phycoerythrin are temperature and moisture removal by dehydration. The latter demonstrates that gentle drying of the biomass allows removing the most significant amount of moisture from the biomass without affecting the concentration or quality of temperature-sensitive proteins.

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Influence of Critical Parameters on the Extraction of Concentrated C-PE from Thermotolerant Cyanobacteria
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Vergel-Suarez, Ariadna H. | 2024-06-24

This work aimed to identify the influence of pH, molarity, w/v fraction, extraction time, agitation, and either a sodium (Na2HPO4 ·7H2O-NaH2PO4 ·H2O) or potassium buffer (K2HPO4 -KH2PO4 ) used in the extraction of C-phycoerythrin (C-PE) from a thermotolerant strain of Potamosiphon sp. An experimental design (Minimum Run Resolution V Factorial Design) and a Central Composite Design (CCD) were used. According to the statistical results of the first design, the K-PO4 buffer, pH, molarity, and w/v fraction are vital factors that enhance the extractability of C-PE. The construction of a CCD design of the experiments suggests that the potassium phosphate buffer at pH 5.8, longer extraction times (50 min), and minimal extraction speed (1000 rpm) are ideal for maximizing C-PE concentration, while purity is unaffected by the design conditions. This optimization improves extraction yields and maintains the desired bright purple color of the phycobiliprotein

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Production of industrial-interest colorants in microalgae and cyanobacteria: leveraging nutrient dynamics and photoperiod optimization
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Contreras Ropero, Jefferson Eduardo | 2024-07-15

The exploration of the biodiversity of cyanobacteria and microalgae holds great promise for obtaining pigments of industrial interest. This study focused on the bioprospecting of these organisms to obtain pigments of industrial interest together with enriched biomass. We investigated the impact of photoperiod and concentration of C/N/P nutrient sources in heterotrophic cultures, controlling specific variables such as light intensity, volume, pH, temperature, air flow and time to produce phycobiliproteins in Arthrospira platensis UTEX1926 and total carotenoids in Scenedesmus sp. using a nonfactorial Plackett‒Burman design in STATISTICA 7.0 software. The biomass concentration was determined by dry weight, while the concentrations of phycocyanin and carotenoids were determined spectrophotometrically at specific wavelengths. Heterotrophic cultures demonstrated superior productivity, with phycocyanin and carotenoids achieving percentages of 7% and 0.4% (w/w), respectively, and an R2 value of 0.99. Among the influential parameters, potassium diphosphate and sodium bicarbonate played pivotal roles in the final deposition of carotenoids and phycocyanin, respectively, yielding concentrations of 96.5 mg/L phycocyanin and 6.5 mg/L carotenoids

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Listar por tema "phycobiliproteins"

Impact of Biomass Drying Process on the Extraction Efficiency of C-Phycoerythrin

Influence of Critical Parameters on the Extraction of Concentrated C-PE from Thermotolerant Cyanobacteria

Production of industrial-interest colorants in microalgae and cyanobacteria: leveraging nutrient dynamics and photoperiod optimization

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Impact of Biomass Drying Process on the Extraction Efficiency of C-Phycoerythrin

Influence of Critical Parameters on the Extraction of Concentrated C-PE from Thermotolerant Cyanobacteria

Production of industrial-interest colorants in microalgae and cyanobacteria: leveraging nutrient dynamics and photoperiod optimization

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Listar por tema "phycobiliproteins"

Impact of Biomass Drying Process on the Extraction Efficiency of C-Phycoerythrin ﻿

Influence of Critical Parameters on the Extraction of Concentrated C-PE from Thermotolerant Cyanobacteria ﻿

Production of industrial-interest colorants in microalgae and cyanobacteria: leveraging nutrient dynamics and photoperiod optimization ﻿

Envíos recientes

Impact of Biomass Drying Process on the Extraction Efficiency of C-Phycoerythrin

Influence of Critical Parameters on the Extraction of Concentrated C-PE from Thermotolerant Cyanobacteria

Production of industrial-interest colorants in microalgae and cyanobacteria: leveraging nutrient dynamics and photoperiod optimization

Portales Institucionales

Impact of Biomass Drying Process on the Extraction Efficiency of C-Phycoerythrin

Influence of Critical Parameters on the Extraction of Concentrated C-PE from Thermotolerant Cyanobacteria

Production of industrial-interest colorants in microalgae and cyanobacteria: leveraging nutrient dynamics and photoperiod optimization