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Effect of the medium composition on the asymbiotic germination and in vitro development of the Laeliocattleya hybrid

SALAZAR MERCADO, SEIR ANTONIO; Botello Delgado, Edinson Alexander (South African Journal of BotanyAmsterdam , Paises Bajos, 2020-12)

In vitro cultivation techniques are viable alternatives for the germination and production of orchid seedlings in a short period. From another perspective, when studying the optimal germination conditions, knowing about ...
Effect of the medium composition on the asymbiotic germination and in vitro development of the Laeliocattleya hybrid

SALAZAR MERCADO, SEIR ANTONIO; Botello Delgado, Edinson Alexander (South African Journal of Botany, 2020-09-04)

In vitro cultivation techniques are viable alternatives for the germination and production of orchid seedlings in a short period. From another perspective, when studying the optimal germination conditions, knowing about ...
Optimization of the tetrazolium test in three species of orchids of the Andean forest

SALAZAR MERCADO, SEIR ANTONIO; Quintero Caleño, Jesús David; Rojas Suárez, Jhan Piero (Australian Journal of Crop ScienceAustralia, 2020-05)

In order to improve the cultivation and conservation of orchid species, it is important to assess the physiological quality of orchid seeds. One of the most traditional tests for analysing seed quality is the tetrazolium ...

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Effect of the medium composition on the asymbiotic germination and in vitro development of the Laeliocattleya hybrid
...
SALAZAR MERCADO, SEIR ANTONIO | 2020-12

In vitro cultivation techniques are viable alternatives for the germination and production of orchid seedlings in a short period. From another perspective, when studying the optimal germination conditions, knowing about the seeds viability is essential. Therefore, this research aimed to study the appropriate medium for germination and in vitro development of xLaeliocattleya Richard Muller. In addition, optimal preconditioning was determined to enhance the tetrazolium test. Initially, the indehiscent capsules were collected in a nursery in Bochalema-Norte de Santander. From them, seeds were obtained for the preconditioning and germination tests. One portion was subjected to different precondition treatments: distilled water, sodium hypochlorite (0.5–1.0%) and sucrose (10%). Afterwards, seeds were rinsed and exposed to different concentrations and exposure periods of tetrazolium. Regarding in vitro cultivation, the commercial medium Orchid Seed Sowing Medium, Murashige & Skoog (MS), and the MS supplemented with coconut water (MSC), pineapple juice (MSP) and gibberellic acid (MSGA3) were evaluated. The highest percentage of viability was obtained by subjecting the seeds to preconditioning with 10% sucrose and keeping them for 24 h in a 0.5% tetrazolium solution. With respect to the MS media used, those enriched with organic supplements proved to be superior to the other treatments since the highest results were achieved both in asymbiotic germination (56 ± 2.8%) and in seedlings formation (25.8 ± 0.8) with the MSP medium, followed by the MSC medium. In this way, a viable and low-cost alternative is offered for the commercial propagation of xLaeliocattleya Richard Muller.

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Effect of the medium composition on the asymbiotic germination and in vitro development of the Laeliocattleya hybrid
...
SALAZAR MERCADO, SEIR ANTONIO | 2020-09-04

In vitro cultivation techniques are viable alternatives for the germination and production of orchid seedlings in a short period. From another perspective, when studying the optimal germination conditions, knowing about the seeds viability is essential. Therefore, this research aimed to study the appropriate medium for germination and in vitro development of xLaeliocattleya Richard Muller. In addition, optimal preconditioning was determined to enhance the tetrazolium test. Initially, the indehiscent capsules were collected in a nursery in Bochalema-Norte de Santander. From them, seeds were obtained for the preconditioning and germination tests. One portion was subjected to different precondition treatments: distilled water, sodium hypochlorite (0.5 1.0%) and sucrose (10%). Afterwards, seeds were rinsed and exposed to different concentrations and exposure periods of tetrazolium. Regarding in vitro cultivation, the commercial medium Orchid Seed Sowing Medium, Murashige & Skoog (MS), and the MS supplemented with coconut water (MSC), pineapple juice (MSP) and gibberellic acid (MSGA3) were evaluated. The highest percentage of viability was obtained by subjecting the seeds to preconditioning with 10% sucrose and keeping them for 24 h in a 0.5% tetrazolium solution. With respect to the MS media used, those enriched with organic supplements proved to be superior to the other treatments since the highest results were achieved both in asymbiotic germination (56 § 2.8%) and in seedlings formation (25.8 § 0.8) with the MSP medium, followed by the MSC medium. In this way, a viable and low-cost alternative is offered for the commercial propagation of xLaeliocattleya Richard Muller.

LEER
Optimization of the tetrazolium test in three species of orchids of the Andean forest
...
SALAZAR MERCADO, SEIR ANTONIO | 2020-05

In order to improve the cultivation and conservation of orchid species, it is important to assess the physiological quality of orchid seeds. One of the most traditional tests for analysing seed quality is the tetrazolium test, whose effectiveness is known to improve with pre-treatment of the seeds. As a result, this study compared various pre-treatments to determine how efficient they were at optimising the tetrazolium test in seeds belonging to Cyrtochilum aemulun, Elleanthus aurantiacus and Lephantes sp. The experiment was based on five different pre-treatment solutions, after which the seeds were exposed to tetrazolium at two different concentrations and for three different exposure times. First, the seeds were soaked for ten minutes in one of five pre-treatment solutions: chlorine 0.5%, chlorine 1%, deionised water, sucrose solution 10% (w/v) or a control in which no pre-treatment was applied. After pre-treatment, the seeds were soaked in the dark in a tetrazolium solution, either at 0.25% concentration or at 1.0% concentration, and for either 6, 24 or 48 hours. The control seeds exposed to no pre-treatment showed an increase in the viability of Lephantes sp. at significant difference from the pre-treated seeds. Similarly, pre-treatment with 10% sucrose produced an increase in the tetrazolium test’s effectiveness in E.aurantiacus seeds, with significant differences from the control in most treatments. Therefore, the use of sucrose as a pre-treatment is recommended, in addition to increasing the concentration and exposure time of the seeds.

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Listar por tema "Viability"

Effect of the medium composition on the asymbiotic germination and in vitro development of the Laeliocattleya hybrid

Effect of the medium composition on the asymbiotic germination and in vitro development of the Laeliocattleya hybrid

Optimization of the tetrazolium test in three species of orchids of the Andean forest

Envíos recientes

Effect of the medium composition on the asymbiotic germination and in vitro development of the Laeliocattleya hybrid

Effect of the medium composition on the asymbiotic germination and in vitro development of the Laeliocattleya hybrid

Optimization of the tetrazolium test in three species of orchids of the Andean forest

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Listar por tema "Viability"

Effect of the medium composition on the asymbiotic germination and in vitro development of the Laeliocattleya hybrid ﻿

Effect of the medium composition on the asymbiotic germination and in vitro development of the Laeliocattleya hybrid ﻿

Optimization of the tetrazolium test in three species of orchids of the Andean forest ﻿

Envíos recientes

Effect of the medium composition on the asymbiotic germination and in vitro development of the Laeliocattleya hybrid

Effect of the medium composition on the asymbiotic germination and in vitro development of the Laeliocattleya hybrid

Optimization of the tetrazolium test in three species of orchids of the Andean forest

Portales Institucionales

Effect of the medium composition on the asymbiotic germination and in vitro development of the Laeliocattleya hybrid

Effect of the medium composition on the asymbiotic germination and in vitro development of the Laeliocattleya hybrid

Optimization of the tetrazolium test in three species of orchids of the Andean forest