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Evaluation of the cytotoxic potential of sodium hypochlorite using meristematic root cells of Lens culinaris Med

SALAZAR MERCADO, SEIR ANTONIO; Maldonado, Hanner Alejandra (Science of The Total EnvironmentPaíses Bajos, 2020-01-20)

The present investigation was designed to monitor the cytotoxic potential of Sodium Hypochlorite using lentil (Lens culinaris) as a bioindicator of toxicity. Sodium hypochlorite (NaClO), is a chemical compound that is used ...
Evaluation of the cytotoxic potential of sodium hypochlorite using meristematic root cells of Lens culinaris Med

SALAZAR MERCADO, SEIR ANTONIO; Maldonado Bayona, Hanner Alejandra (Science Of The Total Environment, 2020-01-20)

The present investigation was designed to monitor the cytotoxic potential of Sodium Hypochlorite using lentil (Lens culinaris) as a bioindicator of toxicity. Sodium hypochlorite (NaClO), is a chemical compound that is used ...
Use of Lens culinaris Med test as environmental bioindicator to identify the cytogenotoxic effect of paraquat pesticide

SALAZAR MERCADO, SEIR ANTONIO; Quintero Caleño, Jesús David (Environmental Science And Pollution ResearchBerlin , Alemania, 2021-05-12)

Paraquat is the most widely used herbicide and the third most sold pesticide in the world, applied in more than 120 countries despite being banned in the European Union. It is a risk to ecosystems. The genotoxic effect of ...

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Evaluation of the cytotoxic potential of sodium hypochlorite using meristematic root cells of Lens culinaris Med
...
SALAZAR MERCADO, SEIR ANTONIO | 2020-01-20

The present investigation was designed to monitor the cytotoxic potential of Sodium Hypochlorite using lentil (Lens culinaris) as a bioindicator of toxicity. Sodium hypochlorite (NaClO), is a chemical compound that is used mainly for its disinfectant properties, its effect is widely toxic, which is why it is marketed in low concentrations and it is also a component in various products such as agrochemicals. In the present study the L. culinaris seeds were exposed to different NaClO dose 0, 0.2, 1, 3, 5 and 7 mg L−1 during 24, 48 and 72 h; timeslots in which the root growth was also studied. The cytotoxic potential of NaClO was determined by calculating the mitotic index (MI), calculating cellular anomalies (CA) and observing the longitudinal growth of the roots during the various time periods. The radicular growth was prolonged and it was observed that there was a greater growth at the dose of 1 and 7 mg L−1 in the time of 72 h. The cytotoxic effects could be analyzed in the mitotic index, since the higher the concentration, the lower the mitotic index, as observed in the dose of 7 mg L−1 where a reduction of the mitotic index of the meristematic cells is observed. The results indicate that NaClO has a cytotoxic effect that induces various types of chromosomal abnormalities. This indicates that Sodium Hypochlorite has a cytotoxic effect according to the increase in its dose. Therefore, Lens culinaris turned out to be a kind of appropriate bioindicator to study the cytotoxic effects of various potentially toxic substances.

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Evaluation of the cytotoxic potential of sodium hypochlorite using meristematic root cells of Lens culinaris Med
...
SALAZAR MERCADO, SEIR ANTONIO | 2020-01-20

The present investigation was designed to monitor the cytotoxic potential of Sodium Hypochlorite using lentil (Lens culinaris) as a bioindicator of toxicity. Sodium hypochlorite (NaClO), is a chemical compound that is used mainly for its disinfectant properties, its effect is widely toxic, which is why it is marketed in low concentrations and it is also a component in various products such as agrochemicals. In the present study the L. culinaris seeds were exposed to different NaClO dose 0, 0.2, 1, 3, 5 and 7 mg L−1 during 24, 48 and 72 h; timeslots in which the root growth was also studied. The cytotoxic potential of NaClO was determined by calculating the mitotic index (MI), calculating cellular anomalies (CA) and observing the longitudinal growth of the roots during the various time periods. The radicular growth was prolonged and it was observed that there was a greater growth at the dose of 1 and 7 mg L−1 in the time of 72 h. The cytotoxic effects could be analyzed in the mitotic index, since the higher the concentration, the lower the mitotic index, as observed in the dose of 7 mg L−1 where a reduction of the mitotic index of the meristematic cells is observed. The results indicate that NaClO has a cytotoxic effect that induces various types of chromosomal abnormalities. This indicates that Sodium Hypochlorite has a cytotoxic effect according to the increase in its dose. Therefore, Lens culinaris turned out to be a kind of appropriate bioindicator to study the cytotoxic effects of various potentially toxic substances.

LEER
Use of Lens culinaris Med test as environmental bioindicator to identify the cytogenotoxic effect of paraquat pesticide
...
SALAZAR MERCADO, SEIR ANTONIO | 2021-05-12

Paraquat is the most widely used herbicide and the third most sold pesticide in the world, applied in more than 120 countries despite being banned in the European Union. It is a risk to ecosystems. The genotoxic effect of paraquat was evaluated using the Lens culinaris test. L. culinaris seeds were subjected to 6 concentrations of paraquat (0.1, 0.5, 1, 1.5, 1.5, 2, and 3 ppm) plus a control (distilled water). During 72 h, root development was measured every 24 h. After 3 days, root apices were analyzed to obtain the inhibition of the mitotic index, as well as the type and rate of chromosomal abnormalities present. A decrease in root growth of more than 50% (72 h of exposure) and an inhibition of the mitotic index of 2.9 times in the treatment with 3 ppm compared to the control were observed. The 2 ppm concentration presented all the anomalies found with a frequency of 84 ± 2.5 of micronuclei, 106 ± 3.5 of nuclear lesions, 14 ± 4.7 of nucleus absence, 8 ± 2.7 of telophase bridges, 7 ± 2.7 of binucleated cells, among others. It is also recommended to establish comparisons of L. culinaris with multiple biomarkers since it is presented as a practical and economic alternative.

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Evaluation of the cytotoxic potential of sodium hypochlorite using meristematic root cells of Lens culinaris Med

Evaluation of the cytotoxic potential of sodium hypochlorite using meristematic root cells of Lens culinaris Med

Use of Lens culinaris Med test as environmental bioindicator to identify the cytogenotoxic effect of paraquat pesticide

Envíos recientes

Evaluation of the cytotoxic potential of sodium hypochlorite using meristematic root cells of Lens culinaris Med

Evaluation of the cytotoxic potential of sodium hypochlorite using meristematic root cells of Lens culinaris Med

Use of Lens culinaris Med test as environmental bioindicator to identify the cytogenotoxic effect of paraquat pesticide

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Listar por tema "Lens culinaris"

Evaluation of the cytotoxic potential of sodium hypochlorite using meristematic root cells of Lens culinaris Med ﻿

Evaluation of the cytotoxic potential of sodium hypochlorite using meristematic root cells of Lens culinaris Med ﻿

Use of Lens culinaris Med test as environmental bioindicator to identify the cytogenotoxic effect of paraquat pesticide ﻿

Envíos recientes

Evaluation of the cytotoxic potential of sodium hypochlorite using meristematic root cells of Lens culinaris Med

Evaluation of the cytotoxic potential of sodium hypochlorite using meristematic root cells of Lens culinaris Med

Use of Lens culinaris Med test as environmental bioindicator to identify the cytogenotoxic effect of paraquat pesticide

Portales Institucionales

Evaluation of the cytotoxic potential of sodium hypochlorite using meristematic root cells of Lens culinaris Med

Evaluation of the cytotoxic potential of sodium hypochlorite using meristematic root cells of Lens culinaris Med

Use of Lens culinaris Med test as environmental bioindicator to identify the cytogenotoxic effect of paraquat pesticide