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Listar por autor "Rodriguez Ordonez, Dora Cecilia"

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    • Alkaline pre-treatment and hydrolysis with acid cellulase of the rice husk (Oryza sativa) for production of bioethanol 

      Cacua Silva, Alejandra; Rodriguez Ordonez, Dora Cecilia; Parra LLanos, John Wilmer (Journal of Physics: Conference SeriesLondres, Reino Unido, 2019-11-19)
      Several companies generate large amounts of agroindustrial waste, which could be used as raw material for the production of bioethanol; among them, those that process and commercialize rice, which produce a large amount ...
    • Analysis of folic acid in white rice by ultra-fast liquid chromatography 

      Rodriguez Ordonez, Dora Cecilia; Torres Herrera, Laura Natalia; Parra LLanos, John Wilmer (Journal of Physics: Conference SeriesLondres, Reino Unido, 2019-11-19)
      The method of analysis was standardized, and the folic acid content was determined in samples of fortified white rice (Z, G and D) and unfortified (E and O). The analysis was performed in triplicate in samples of 2.0000 ...
    • Production of bioethanol from rice husk pretreated with alkalis and hydrolyzed with acid cellulase at pilot scale 

      Cacua Silva, Alejandra; Rodriguez Ordonez, Dora Cecilia; Parra LLanos, John Wilmer (Journal of Physics: Conference Series, 2018-12-07)
      Rice husk (RH) is the main biomass byproduct of the rice industry, so much that it corresponds to 20% of the total cereal production and is generally considered a waste. Due to its content of cellulose and hemicellulose, ...

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      • Portada Alkaline pre-treatment and hydrolysis with acid cellulase of the rice husk (Oryza sativa) for production of bioethanol

        Alkaline pre-treatment and hydrolysis with acid cellulase of the rice husk (Oryza sativa) for production of bioethanol

        ...

        Cacua Silva, Alejandra | 2019-11-19

        Several companies generate large amounts of agroindustrial waste, which could be used as raw material for the production of bioethanol; among them, those that process and commercialize rice, which produce a large amount of husk, a lignocellulosic material, as a by-product. These companies generally use rice husk as fuel for the ovens, generating problems to the environment due to the emission of polluting smoke. The objective of this work was to obtain bioethanol from rice husk, taking advantage of its cellulose and hemicellulose content. The lignin was removed by an alkaline pretreatment with 2.00% w/v sodium hidroxide solution. Cellulose and hemicellulose in the husk were hydrolysed by enzymatic hydrolysis with acid cellulase for 72 hours. During this period of time, the concentration of total reducing sugars was monitored by the 3,5-dinitrosalicylic acid method, in order to determine the reaction time in which the highest concentration of total reducing sugars is obtained. The sugars obtained were fermented with saccharomyces cerevisiae, for the production of alcohol. During the enzymatic hydrolysis of the pre-treated rice husk, it was found that the highest concentration of total reducing sugars in the samples was 7.778 g/L, obtained in a time of 24 hours of hydrolysis. The sugars obtained were fermented with Saccharomyces cerevisiae, for alcohol production. A distillate was obtained with a concentration of 3.7% v/v.

        LEER

      • Portada Analysis of folic acid in white rice by ultra-fast liquid chromatography

        Analysis of folic acid in white rice by ultra-fast liquid chromatography

        ...

        Rodriguez Ordonez, Dora Cecilia | 2019-11-19

        The method of analysis was standardized, and the folic acid content was determined in samples of fortified white rice (Z, G and D) and unfortified (E and O). The analysis was performed in triplicate in samples of 2.0000 g, hydrolysis was performed with phosphate buffer pH 8.82 for one hour and then lowered to pH 7.00 for enzymatic treatment with takadiastase at 65°C. The extracts were filtered by gravity, purified with nylon syringe filters and ion exchange cartridges (solid phase extraction using strong anion exchange cartridges). The concentration in mg folic acid/kg of rice, in each extract was determined by ultrafast liquid chromatography, with average values for Lot1 of the fortified rice samples, of 44.03, 51.95, 38.08, and unfortified samples of 31.51 and 21.94, and with respect to Lot2 of 41.26, 41.27, 34.54 for fortified ones and 24.92 and 20.94 for non-fortified ones, respectively. The concentration of folic acid obtained by filtration through syringe filters was higher than in the extracts purified by solid phase extraction using strong anion exchange cartridges, evidencing loss of the analyte. The recovery of folic acid in the samples doped and purified by syringe filters was around 65.00%, while by solid phase extraction using strong anion exchange cartridges 43.00%. There is evidence of variation in the folic acid content between different batches of rice of the same brand.

        LEER

      • Portada Production of bioethanol from rice husk pretreated with alkalis and hydrolyzed with acid cellulase at pilot scale

        Production of bioethanol from rice husk pretreated with alkalis and hydrolyzed with acid cellulase at pilot scale

        ...

        Cacua Silva, Alejandra | 2018-12-07

        Rice husk (RH) is the main biomass byproduct of the rice industry, so much that it corresponds to 20% of the total cereal production and is generally considered a waste. Due to its content of cellulose and hemicellulose, the RH has a great potential for its alternative use in the production of bioethanol, since it has a great advantage with respect to the conventional raw materials used (sugar cane, corn, beet and sweet sorghum, among others), given that this lignocellulosic material is abundant and does not compete with the food industry. The objective of this work was to obtain bioethanol from rice husk at a pilot scale, taking advantage of its cellulose and hemicellulose content. For this, a sample of 450g of RH was taken, which was pretreated with a 2.00% w/v NaOH solution and subsequently hydrolyzed with acid cellulase (CFB3S). The alcoholic fermentation of total reducing sugars (TRS) obtained in the enzymatic hydrolysis was carried out with Saccharomyces cerevisiae, finally, the fractional distillation of the must was carried out, by means of which four bioethanol samples were obtained with concentrations of 71.3% v/v, 30.0% v/v, 10.3% v/v, and 5.3% v/v of alcohol, which when mixed produced a sample of 95mL of solution with a concentration of 27% v/v alcohol (4.70g/100g of RH).

        LEER

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