Show simple item record

dc.contributor.authorSALAZAR MERCADO, SEIR ANTONIO
dc.contributor.authorRojas Suárez, Jhan Piero
dc.contributor.authorTorres-León, Carlos Andrés
dc.date.accessioned2021-12-09T20:07:26Z
dc.date.available2021-12-09T20:07:26Z
dc.date.issued2019-05-30
dc.identifier.urihttp://repositorio.ufps.edu.co/handle/ufps/1777
dc.description.abstractThe objective of this study was to evaluate the cytotoxic effect of different sodium hypochlorite concentrations, using apical root cells of P. sativum as a bioindicator. Initially, the seeds of P. sativum were exposed to different concentrations of sodium hypochlorite (0.1, 0.2, 0.4, 0.8, 1.6, 2 mg L−1) and to a control solution based on distilled water. Next, root growth was measured during 24, 48 and 72 h. Subsequently, the mitotic index (MI) and cellular anomalies (5000 cells per treatment) were determined at 72 h. According to the results obtained, a decrease in root growth was observed at concentrations of 0.4, 1.6 and 2 mg L−1. Likewise, it was evident that, among all the evaluated concentrations, an inhibition of mitosis higher than 50% was presented. Additionally, chromosomal anomalies were also generated, such as Nuclear notch, lagging chromosomes and Chromosomal break, which were present in all the concentrations evaluated. In addition, the presence of micronuclei at concentrations of 2.0 and 1.6 mg L−1 indicate that sodium hypochlorite is a highly cytotoxic substance. Therefore, P. sativum is a specie that offers a feasible experimental model to be implemented in the laboratory with the aim to evaluate the cytotoxic effect of any cytotoxic substance.eng
dc.format.mimetypeapplication/pdfspa
dc.language.isoengspa
dc.publisherEcotoxicology and Environmental Safetyspa
dc.relation.ispartofEcotoxicology and Environmental Safety
dc.rights© 2019 Elsevier Inc. All rights reserved.eng
dc.sourcehttps://www.sciencedirect.com/science/article/abs/pii/S0147651319301770spa
dc.titleCytotoxic evaluation of sodium hypochlorite, using Pisum sativum L as effective bioindicatoreng
dc.typeArtículo de revistaspa
dc.identifier.doihttps://doi.org/10.1016/j.ecoenv.2019.02.027
dc.publisher.placeWashintong D.C , Estados Unidosspa
dc.relation.citationeditionVol.173 (2019)spa
dc.relation.citationendpage76spa
dc.relation.citationstartpage71spa
dc.relation.citationvolume173spa
dc.relation.citesSalazar-Mercado, S. A., Torres-León, C. A., & Rojas-Suárez, J. P. (2019). Cytotoxic evaluation of sodium hypochlorite, using Pisum sativum L as effective bioindicator. Ecotoxicology and environmental safety, 173, 71-76.
dc.relation.ispartofjournalEcotoxicology and Environmental Safetyspa
dc.rights.accessrightsinfo:eu-repo/semantics/restrictedAccessspa
dc.subject.proposalCellular abnormalitieseng
dc.subject.proposalCytotoxicityeng
dc.subject.proposalSodium hypochloriteeng
dc.subject.proposalMitotic indexP. sativumeng
dc.type.coarhttp://purl.org/coar/resource_type/c_6501spa
dc.type.contentTextspa
dc.type.driverinfo:eu-repo/semantics/articlespa
dc.type.redcolhttp://purl.org/redcol/resource_type/ARTspa
oaire.accessrightshttp://purl.org/coar/access_right/c_14cbspa
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85spa
dc.type.versioninfo:eu-repo/semantics/publishedVersionspa


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record