TY - NEWS
TI - Optimization of Phycobiliprotein Solubilization from a Thermotolerant Oscillatoria sp
AU - Barajas Solano, andres F
AB - The present study evaluated the effect of multiple variables (drying time, drying temperature, biomass/solvent ratio, glass beads/biomass ratio, extraction time, and extraction speed) in the
solubilization of three different phycobiliproteins (C-PC, APC, and PE) from a thermotolerant Oscillatoria sp. The strain was grown in BG11 media (28 ◦C, light: dark cycle of 12:12 h at 100 µmol·m−2
·s
−1
,
20 days) and the experiments were conducted according to a two-level randomized factorial design
with six center points (38 runs). Results show that biomass/solvent ratio, glass beads/biomass ratio,
and extraction time, are the most significant variables in the extraction of all three proteins, whereas
the glass beads/biomass ratio and extraction time significantly affect their purity. The optimized
conditions allow a statistical increase in the concentration of C-PC, APC, and PE extracted from the
biomass; however, the purity was lower in comparison with the expected value. The latter occurs
due to a larger biomass/solvent ratio and longer extraction times, which enhanced the solubility of
other hydrophilic metabolites (proteins and carbohydrates, etc.).
DA - 2022-04-24
KW - surface response methodology
KW - allophycocyanin
KW - phycocyanin
KW - phycoerythrin
KW - drying
KW - biomass/solvent ratio
KW - cell lysis
PB - Processes
UR - https://repositorio.ufps.edu.co/handle/ufps/6535
ER -