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Optimization of Phycobiliprotein Solubilization from a Thermotolerant Oscillatoria sp
dc.contributor.author | Barajas Solano, andres F | |
dc.date.accessioned | 2022-11-18T14:18:02Z | |
dc.date.available | 2022-11-18T14:18:02Z | |
dc.date.issued | 2022-04-24 | |
dc.identifier.uri | https://repositorio.ufps.edu.co/handle/ufps/6535 | |
dc.description.abstract | The present study evaluated the effect of multiple variables (drying time, drying temperature, biomass/solvent ratio, glass beads/biomass ratio, extraction time, and extraction speed) in the solubilization of three different phycobiliproteins (C-PC, APC, and PE) from a thermotolerant Oscillatoria sp. The strain was grown in BG11 media (28 ◦C, light: dark cycle of 12:12 h at 100 µmol·m−2 ·s −1 , 20 days) and the experiments were conducted according to a two-level randomized factorial design with six center points (38 runs). Results show that biomass/solvent ratio, glass beads/biomass ratio, and extraction time, are the most significant variables in the extraction of all three proteins, whereas the glass beads/biomass ratio and extraction time significantly affect their purity. The optimized conditions allow a statistical increase in the concentration of C-PC, APC, and PE extracted from the biomass; however, the purity was lower in comparison with the expected value. The latter occurs due to a larger biomass/solvent ratio and longer extraction times, which enhanced the solubility of other hydrophilic metabolites (proteins and carbohydrates, etc.). | eng |
dc.format.extent | 12 | spa |
dc.format.mimetype | application/pdf | spa |
dc.language.iso | eng | spa |
dc.publisher | Processes | spa |
dc.relation.ispartof | Processes. vol 10 No°5[2022] | |
dc.rights | © 2022 by the author | eng |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | spa |
dc.source | https://www.scopus.com/record/display.uri?eid=2-s2.0-85129605858&doi=10.3390%2fpr10050836&origin=inward&txGid=0e19ef6bd30a064ef3585ac87387ecfe | spa |
dc.title | Optimization of Phycobiliprotein Solubilization from a Thermotolerant Oscillatoria sp | eng |
dc.type | Artículo de revista | spa |
dc.contributor.corporatename | Processes | spa |
dc.identifier.doi | https://doi.org/10.3390/pr10050836 | |
dc.publisher.place | Suiza | spa |
dc.relation.citationedition | Vol. 10 No° 5 [2022] | spa |
dc.relation.citationendpage | 12 | spa |
dc.relation.citationissue | 5[2022] | spa |
dc.relation.citationstartpage | 1 | spa |
dc.relation.citationvolume | 10 | spa |
dc.relation.cites | Barajas-Solano, A.F. Optimization of Phycobiliprotein Solubilization from a Thermotolerant Oscillatoria sp. Processes 2022, 10, 836. https://doi.org/10.3390/pr10050836 | |
dc.relation.ispartofjournal | Processes | spa |
dc.rights.accessrights | info:eu-repo/semantics/openAccess | spa |
dc.rights.creativecommons | Atribución 4.0 Internacional (CC BY 4.0) | spa |
dc.subject.proposal | surface response methodology | eng |
dc.subject.proposal | allophycocyanin | eng |
dc.subject.proposal | phycocyanin | eng |
dc.subject.proposal | phycoerythrin | eng |
dc.subject.proposal | drying | eng |
dc.subject.proposal | biomass/solvent ratio | eng |
dc.subject.proposal | cell lysis | eng |
dc.type.coar | http://purl.org/coar/resource_type/c_2df8fbb1 | spa |
dc.type.content | Text | spa |
dc.type.driver | info:eu-repo/semantics/article | spa |
dc.type.redcol | http://purl.org/redcol/resource_type/ART | spa |
oaire.accessrights | http://purl.org/coar/access_right/c_abf2 | spa |
oaire.version | http://purl.org/coar/version/c_970fb48d4fbd8a85 | spa |
dc.type.version | info:eu-repo/semantics/publishedVersion | spa |
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