Fito bioquímica y Biología Molecular - FITOBIOMOLhttps://repositorio.ufps.edu.co/handle/ufps/842024-03-29T05:12:29Z2024-03-29T05:12:29ZIn Vitro antifungal activity of ethanol extracts from Cnidoscolus urens L. in controlling Colletotrichum spp. in Lycopersicum esculentum: a sustainable agricultural perspectiveCarrillo, Maria FernandaMora Estupiñan, Daniela AlejandraOrtiz, LuzChaves-Bedoya, Giovannihttps://repositorio.ufps.edu.co/handle/ufps/67682024-03-23T08:00:40Z2023-05-01T00:00:00ZIn Vitro antifungal activity of ethanol extracts from Cnidoscolus urens L. in controlling Colletotrichum spp. in Lycopersicum esculentum: a sustainable agricultural perspective
Carrillo, Maria Fernanda; Mora Estupiñan, Daniela Alejandra; Ortiz, Luz; Chaves-Bedoya, Giovanni
Anthracnose, caused by the pathogenic fungi Colletotrichum spp., poses a significant threat to table tomato (Lycopersicum esculentum) cultivation. This study delves into the potential of plant extracts from Cnidoscolus urens L. as an alternative biocontrol strategy to combat this disease. Rich in secondary metabolites like terpenes, which are instrumental in plant defense, these extracts also comprise esters and fatty acids. Although the latter are not classified as secondary metabolites, they contribute significantly to the plant's biochemical makeup. Our objective was to gauge the in vitro inhibitory efficacy of ethanolic extracts derived from the leaves and stems of Cnidoscolus urens L. against Colletotrichum spp. To achieve this, an agar dilution method with varying extract concentrations was employed. The results showed that concentrations ranging from treatment 3 to treatment 8 effectively inhibited fungal mycelial growth. Interestingly, the extracts' origin, whether from leaves or stems, did not show any significant differential impact on their inhibitory activity. These insights emphasize the consistent effect of Cnidoscolus urens L. extracts in stalling Colletotrichum spp. growth, underscoring their potential as biological antifungal agents in agriculture. Given the pronounced in vitro effectiveness of both leaf and stem extracts, they beckon further exploration as part of sustainable agricultural strategies to combat prominent diseases like anthracnose.
2023-05-01T00:00:00ZSilver colloidal nanoparticles by electrochemistry: temporal evaluation and surface plasmon resonancePadilla Sierra, H APeña Rodríguez, GChaves Bedoya, Giovannihttps://repositorio.ufps.edu.co/handle/ufps/65892022-11-23T08:00:39Z2021-06-04T00:00:00ZSilver colloidal nanoparticles by electrochemistry: temporal evaluation and surface plasmon resonance
Padilla Sierra, H A; Peña Rodríguez, G; Chaves Bedoya, Giovanni
The electrochemical technique for obtaining silver nanoparticles has advantages over
other methods. For the synthesis, a colloidal silver generator (Colloidal Silver Generator® model
1001) was used, where two electrodes coupled to high purity silver rods (99.99%) were used,
with a potential difference of 24 V. Nanoparticle concentration was measured by total dissolved
solids, using the SI-Analytic HandyLab 680 FK multiparameter in 200 mL of Milli-Q deionized
water, reporting 18 ppm at 1 hour at room temperature. The determination of the resonance
wavelength of the surface plasmons was carried out by finding the maximum absorbance by UVVisible spectrophotometry with λ = 423 nm. The morphology and size of the nanoparticles was
determined by Transmission Electron Microscopy, observing spherical morphology and sizes
smaller than 50 nm. The chemical composition was determined by X-ray energy dispersed
spectroscopy, finding a weight concentration of 93.22% of silver. The results show an optimal
synthesis of colloidal silver, with characteristics that will allow the inhibition of microorganisms
of interest.
2021-06-04T00:00:00ZMolecular characterization of two papaya ringspot virus isolates that cause devastating symptoms in Norte de Santander, ColombiaChaves-Bedoya, GiovanniOrtiz, Luzhttps://repositorio.ufps.edu.co/handle/ufps/18142022-05-23T15:54:18Z2017-01-06T00:00:00ZMolecular characterization of two papaya ringspot virus isolates that cause devastating symptoms in Norte de Santander, Colombia
Chaves-Bedoya, Giovanni; Ortiz, Luz
Papaya ringspot virus is an RNA virus that belongs to the genus Potyvirus, family Potyviridae and affects both papaya and cucurbits, causing great economic losses. PRSV isolates are divided into biotypes P and W; both biotypes naturally infect plants in the family Cucurbitaceae, whereas the P type also naturally infects papaya (Carica papaya L). In the present study, we report the full-length genome sequence of two PRSV-P isolates sampled from the Campo Hermoso (PRSV-CH) and Villa del Rosario (PRSV-VR) localities in Norte de Santander, Colombia. The genomes of these PRSV isolates are 10,326 nt in length and have a predicted ORF of 3344 aa. The identity among Colombian PRSV isolates is 96.9% and 97.3% at the nucleotide and deduced amino acid levels, respectively. PRSV isolates from China had the lowest identity at 78.3% and 89.2% (nucleotide-amino acid), whereas the highest identities were detected in PRSV isolates from Mexico, Venezuela and Hawaii. At the polyprotein level, the amino acid composition surrounding the active polyprotein cleavage sites differ in the Colombian PRSV sequences. The predicted cleavage site in P1/HC-Pro is LEQY/N – LEQY/S instead of MEQY/N. Both of the Colombian PRSV isolates have a putative recombination event in the P1 coding region, which is common in all PRSV isolates from the American continent. The new full-length PRSV sequences from Colombia provide a better understanding of the dynamics of papaya ringspot virus infections in papaya in Colombia and worldwide.
2017-01-06T00:00:00ZTranscriptional changes involved in kumquat (Fortunella spp) defense response to Xanthomonas citri subsp. citri in early stages of infectionGIRALDO GONZALEZ, JHON JAIROCarvalho, flávia Maria de SouzaHirochi Herai, RobertoAparecido Ferro, JesusChaves Bedoya, GiovanniRodas Mendoza, Elkin Fernandohttps://repositorio.ufps.edu.co/handle/ufps/18092022-05-23T15:54:15Z2021-12-01T00:00:00ZTranscriptional changes involved in kumquat (Fortunella spp) defense response to Xanthomonas citri subsp. citri in early stages of infection
GIRALDO GONZALEZ, JHON JAIRO; Carvalho, flávia Maria de Souza; Hirochi Herai, Roberto; Aparecido Ferro, Jesus; Chaves Bedoya, Giovanni; Rodas Mendoza, Elkin Fernando
Xanthomonas citri subsp. citri (Xcc), the causal agent of type A canker, differentially affects all commercial varieties of citrus plants. Among them, Fortunella spp (kumquat) have higher tolerance to disease development, although the molecular mechanisms involved in their defense are not fully understood. In this study, using RNA-seq approach, the transcriptional responses of kumquat leaves at 24 h after water (controls) and Xcc inoculation were analyzed. A total of 1439 Differentially expressed genes (DEG) with statistical significance (p-value<0.025) were identified, with 444 being upregulated. These genes were found to be involved in pathogen recognition, cell wall remodeling and reinforcement, lignin biosynthesis, reactive oxygen species (ROS) production, pathogenesis-related proteins (PR) and biosynthesis of secondary metabolites including phenylpropanoids, terpenoids and alkaloids. Genes related to disease resistance (R genes) and salicylic acid (SA)-dependent systemic acquired resistance (SAR) were also induced. In turn, the 995 downregulated genes were mainly associated with photosynthesis, photorespiration, chlorophyll synthesis and cell growth. This suggest that, once the pathogen is detected, the plant generates a strong oxidative burst and its cellular machinery is directed towards the synthesis of secondary metabolites and defense proteins while its own growth is inhibited. Overall, these transcriptional changes provide valuable information about the molecular basis of the defense in kumquat plants, which may be useful in the design of new control methods for citrus canker.
2021-12-01T00:00:00Z