Cellulase Production from Trichoderma harzianum: a Framework Modeling for Evaluating Different Operating Mode Strategies
Mora, D | 2021-09-12
The cellulase enzyme has been used in essential applications in the textile industry
and the formulation of some medicines. Therefore, a mathematical model based on previous
reports is proposed for cellulase enzyme enhancement. First, the kinetic constants were taken
from the literature. Then, two operating strategies were carried out to maximize cellulase
production using 10g/L of cellulose and 0.4g/L of cells as a first strategy using a batch mode.
Alternatively, a second operating mode is simulated using continuous cellulose feeding.
Finally, a comparative analysis was made for determining the best operating mode related to
maximal cellulase production. Interestingly, the continuous mode shows a high cellulase
concentration lasting a shorter time than the batch mode to avoid inhibiting enzyme
production. One of the most important findings of this work focuses on increasing cellulase
production with values of 1800 FPT/L simulating the continuous mode. The latter suggests that
cellulase production could be double the concentration obtained with the traditional batch
mode (800FPU/L). Results obtained in this research may be promising for the enzyme
production industry. Using simulation techniques, it is possible to determine an enhancement
without requiring excessive experimentation and resources.